표제지
목차
국문 초록 12
I. 서론 14
I-1. 연구 배경 14
I-2. 플라즈마 중합에 대한 선행연구 24
II. 실험 34
II-1. 시약 및 분석기기 34
II-2. 플라즈마 중합에 의한 이온성 (쯔비터 이온 및 양이온성) 고분자 기반의 엔자이메틱 바이오센서 제조 36
III. 결과 및 고찰 38
III-1. 플라즈마 중합에 의한 쯔비터 이온 고분자 기반의 엔자이메틱 바이오센서 제조 및 특성평가 38
III-2. 플라즈마 중합에 의한 양이온성 고분자 기반의 엔자이메틱 바이오센서 제조 및 특성평가 63
IV. 결론 88
참고문헌 90
ABSTRACT 106
Table 1. Comparison of the reported glucose biosensors 32
Table 2. Experimental condition of the zwitterion conductive polymer coated electrode preparation 41
Table 3. Interference effects of the prepared enzymatic biosensor to various compounds 59
Table 4. Comparison of the reported glucose biosensors 61
Table 5. Preparation of the coating solution for AC plasma polymerization onto ITO electrode surface 66
Table 6. Interference effects of the prepared enzymatic biosensor to various compounds 84
Table 7. Comparison of the reported glucose biosensors and the fabricated biosensor in this study 86
Figure 1. Electrochemical biosensor for detecting various target molecules. 17
Figure 2. Chart of 2D materials for electrochemical sensor and optical sensor. 23
Figure 3. Schematic preparation of polyaniline Ni-complex catalytic electrode by plasma process for detection of phosphate. 26
Figure 4. Schematic preparation procedure of electrochemical catalytic electrode by AC plasma deposition. 28
Figure 5. Schematic preparation of the Ni-modified catalytic electrode by cold plasma process for MeOH oxidation. 30
Figure 6. Schematic diagrams of the AC plasma deposition process and device. 37
Figure 7. Schematic preparation procedure of enzymatic biosensor by plasma polymerization. 39
Figure 8. ¹H-NMR spectrum of the synthesized SBVI in D₂O. 43
Figure 9. FT-IR spectra of the zwitterion polymeric electrode. 45
Figure 10. Contact angles of the zwitterion polymeric electrode surface (see, Table 1). 47
Figure 11. SEM images of the zwitterion polymeric electrode surface (see, Table 1). 49
Figure 12. AFM images of the zwitterion polymeric electrode surface (see, Table 1). 51
Figure 13. Cyclic voltammograms of 1 mM K3Fe(CN)6 and K4Fe(CN)6 using the zwitter ion polymeric electrode in 0.1 M PBS with scan rate...[이미지참조] 53
Figure 14. Sensing range of the prepared enzymatic biosensor in 0.1MPBS solution with different H₂O₂ concentration at scan rate of 0.1 mV/sec. 55
Figure 15. Chronoamperometry response of the prepared enzymatic biosensor to glucose concentration in 0.1 M PBS solution at a 0.75 V. 57
Figure 16. Schematic preparation procedure of glucose oxidase-modified biosensor based on poly(maleic acid)-modified ITO electrode... 64
Figure 17. Photo and FT-IR spectrum of homopolymers of poly(maleic acid). 68
Figure 18. DSC curve of the maleic anhydride (a) and poly(maleic acid) (b). 70
Figure 19. Contact angles of poly(maleic acid)-coated ITO electrode surface by plasma polymerization. 72
Figure 20. AFM images of poly(maleic acid)-coated ITO electrode (No.1,2,3,4,5) by plasma polymerization. 74
Figure 21. Cyclic voltammograms of 1 mM K3Fe(CN)6 and K4Fe(CN)6 using the poly(maleic acid)-coated ITO electrode in 0.1 M...[이미지참조] 76
Figure 22. SEM images of the No.1, No.4, No.5, and No.5 with GOx as enzymatic biosensor. 78
Figure 23. Sensing range of the prepared enzymatic biosensor in 0.1M PBS solution with different H₂O₂ at scan rate of 100mV/sec. 80
Figure 24. Chronoamperometry response of the prepared enzymatic biosensor to glucose concentration in 0.1M PBS solution at a -0.3 V. 82