표제지
목차
국문초록 17
CHAPTER I. Introduction 19
CHAPTER II. A study on the effect of whitening, wrinkle improvement, tran-2-nonenal removal, and anti-obesity effect of wheat germ hydrothermal extract 32
2-1. Introduction 33
2-2. Experimental 38
2-2-1. 재료 38
2-2-2. 밀 배아 추출조건 38
2-2-3. 밀 배아 추출물의 색차계 측정 40
2-2-4. 밀 배아 추출물에 대한 GC-MS 분석 40
2-2-5. 밀 배아 추출물에 대한 DPPH 소거능 분석 40
2-2-6. 밀 배아 추출물의 Tyrosinase 저해율 분석 41
2-2-7. 밀 배아 추출물의 Collagenase 저해율 분석 42
2-2-8. 밀 배아 추출물 활성성분의 Trans-2-nonenal 제거효능 분석 42
2-2-9. 밀 배아 추출물의 Lipase 활성 저해능 분석 43
2-2-10. 밀 배아 추출물의 α-Glucosidase 활성 저해능 분석 44
2-3. Results and discussion 48
2-3-1. 밀 배아 추출물의 수득율 확인 결과 48
2-3-2. 밀 배아 추출물의 색차계 측정결과 50
2-3-3. 밀 배아 추출물 활성성분의 GC-MS 분석결과 52
2-3-4. 밀 배아 추출물의 항산화 효능 분석결과 61
2-3-5. 밀 배아 추출물의 Tyrosinase 저해활성 분석결과 68
2-3-6. 밀 배아 추출물의 Collagenase 저해활성 분석 결과 70
2-3-7. 밀 배아 추출물 활성성분의 Trans-2-nonenal 제거효능 분석결과 72
2-3-8. 밀 배아 추출물의 Lipase 활성 저해능 분석결과 78
2-3-9. 밀 배아 추출물의 α-Glucosidase 활성 저해능 분석결과 80
2-4. Conclusion 82
CHAPTER III. Comparisons on antibacterial and anti-inflammatory activities of wheat germ extract according to supercritical and solvent extraction methods 84
3-1. Introduction 85
3-2. Experimental 88
3-2-1. 재료 88
3-2-2. 밀 배아에 대한 추출조건 88
3-2-3. 밀 배아 추출물에 대한 수득율 및 DMBQ의 HPLC 분석 89
3-2-4. Oxygen radical absorbance capacity (ORAC) assay에 의한 밀 배아 추출물의 항산화 활성 분석 92
3-2-5. Propionibacterium acnes (P. acnes)에 대한 항균활성 평가 92
3-2-6. 피부 각질세포에서의 염증 완화 효능 평가 93
3-3. Results and discussion 94
3-3-1. 밀 배아 추출물에 대한 수득율 및 DMBQ의 HPLC 분석결과 94
3-3-2. Oxygen radical absorbance capacity (ORAC) assay에 의한 밀 배아 추출물의 항산화 활성 분석 결과 99
3-3-3. Propionibacterium acnes (P. acnes)에 대한 항균활성 평가결과 102
3-3-4. 피부 각질세포에서의 염증 완화 효능 평가 결과 105
3-4. Conclusion 108
References 109
ABSTRACT 124
〈Table 1-1〉 Aldehydes formed by the oxidative degradation of several sebaceous components 26
〈Table 2-1〉 Yield table for wheat germ extracts 49
〈Table 2-2〉 Colorimetric results table of wheat germ extracts 51
〈Table 2-3〉 GC-MS chromatogram profile of 20 % ethanol of wheat germ 56
〈Table 2-4〉 GC-MS chromatogram profile of 50 % ethanol of wheat germ 57
〈Table 2-5〉 GC-MS chromatogram profile of 80 % ethanol of wheat germ 58
〈Table 2-6〉 Confirmation of GC-MS active ingredient analysis results 60
〈Table 2-7〉 Half maximal inhibitory concentration (IC50)[이미지참조] 62
〈Table 2-8〉 UV/Vis results for evaluation of Trans-2-nonenal removal efficacy of extracts by extraction conditions 73
〈Table 2-9〉 GC-MS Results for evaluation of Trans-2-nonenal removal efficacy of extracts by extraction Conditions 76
〈Table 3-1〉 Conditions for analysis of DMBQ content of wheat germ extract using HPLC 91
〈Table 3-2〉 Results of the comparison of the DMBQ content and extraction yield based on the extraction method 98
〈Table 3-3〉 ORAC value result of wheat germ extract 101
〈Table 3-4〉 Inhibition activity (%) of DMBQ and wheat germ extracts 103
〈Figure. 1-1〉 Wrinkle Generation Mechanism. 24
〈Figure. 1-2〉 Melanin production mechanism. 25
〈Figure. 1-3〉 Mechanism of generation of Trans-2-nonenal. 27
〈Figure. 1-4〉 Fat digestion and absorption. 28
〈Figure. 2-1〉 Extraction process of hydrothermal extract. 39
〈Figure. 2-2〉 Classification of Carbohydrates 46
〈Figure. 2-3〉 Digestion of carbohydrates: Breakdown and Absorption 47
〈Figure. 2-4〉 GC-MS chromatogram of 20 % ethanol extracts of wheat germ. 53
〈Figure. 2-5〉 GC-MS chromatogram of 50 % ethanol extracts of wheat germ. 54
〈Figure. 2-6〉 GC-MS chromatogram of 80 % ethanol extracts of wheat germ. 55
〈Figure. 2-7〉 Ascorbic acid antioxidant experiment result graph. 63
〈Figure. 2-8〉 20 % ethanol extract antioxidant experiment result graph. 64
〈Figure. 2-9〉 50 % ethanol extract antioxidant experiment result graph. 65
〈Figure. 2-10〉 80 % ethanol extract antioxidant experiment result graph. 66
〈Figure. 2-11〉 Antioxidant ability according to extraction conditions. 67
〈Figure. 2-12〉 Effect of wheat germ extracts on tyrosinase inhibitory activity. 69
〈Figure. 2-13〉 Effect of wheat germ extracts on collagenase inhibitory activity. 71
〈Figure. 2-14〉 Comparison of UV/Vis results for the evaluation of trans-2-nonenal removal efficacy of extracts according... 74
〈Figure. 2-15〉 GC-MS Results for evaluation of Trans-2-nonenal removal efficacy of extracts by extraction conditions. 77
〈Figure. 2-16〉 Effect of wheat germ extracts on lipase inhibitory activity. 79
〈Figure. 2-17〉 Effect of wheat germ extracts on α-glucosidase inhibitory activity. 81
〈Figure. 3-1〉 DMBQ analysis conditions and HPLC profile (UV: 275 nm). 95
〈Figure. 3-2〉 Calibration curve for standard DMBQ. 96
〈Figure. 3-3〉 DMBQ content analysis graph of wheat germ extract by HPLC. 97
〈Figure. 3-4〉 Peroxyl radical-scavenging activity of wheat germ extract according to the extraction method. 100
〈Figure. 3-5〉 Inhibition activity (%) of DMBQ and wheat germ extracts. 104
〈Figure. 3-6〉 Cell viability of HaCaT cells according to the concentration of wheat germ extract. 106
〈Figure. 3-7〉 Chemokine inhibitory activity of HaCaT cells based on the concentration of wheat germ extract. 107