표제지
목차
국문요약 9
Ⅰ. 서론 16
Ⅱ. 재료 및 방법 20
1. 공시충 및 실내 사육 조건 20
1.1. 상륙 상자 환경 및 조건 21
2. 생육특성 조사 24
2.1. 난기간 조사 24
2.2. 유충의 령기 조사 25
3. 대량 사육 기술 조사 27
3.1. 최적 산란조건 조사 27
3.2. 기존 사육 방법의 동면 전 생존율 조사 28
3.3. 기존 사육 방법의 동면 후 생존율과 성충의 우화율 조사 31
3.4. 자동 물 교환 시스템 적용 방법의 동면 전 생존율 조사 32
3.5. 자동 물 교환 시스템 적용 방법의 동면 후 생존율과 성충의 우화율 조사 38
4. 유충의 저온 저장 가능성 조사 39
4.1. 동면 기간별 생존율과 우화율 조사 39
Ⅲ. 결과 40
1. 생육특성 조사 40
1.1. 난기간 조사 40
1.2. 유충의 령기 조사 41
2. 사육 방법별 생존율과 우화율 43
2.1. 최적 산란조건 조사 43
2.2. 기존 사육방법의 생존율과 우화율 조사 44
2.3. 자동 물 교환 시스템 적용 방법의 생존율과 우화율 조사 45
3. 유충의 저온 저장 가능성 조사 47
3.1. 처리기간별 동면 유충의 생존율과 우화율 47
Ⅳ. 고찰 48
Ⅴ. 참고문헌 50
ABSTRACT 53
Table 1. Comparison of emergence rate of the Luciola lateralis in 2019 22
Table 2. Egg period and hatchability of Luciola lateralis 40
Table 3. Larval period and survival rate at each instar that of the Luciola lateralis in different breeding containers before hibernation 41
Table 4. Hibernation larva instar of the Luciola lateralis in different breeding containers 42
Table 5. Comparison of oviposited numbers of the Luciola lateralis between felt and nonwoven fabric 43
Table 6. Survival number and rates of the Luciola lateralis from each period in breeding container(Ø310×95mm) in 2020~2021 44
Table 7. Survival number and rate of the Luciola lateralis from each period in breeding container(460×319×244mm) in 2021~2022 45
Table 8. Survival number and rate of the Luciola lateralis different rearing methods 46
Table 9. Adults emergence-rates and period of 300 larvae of the Luciola lateralis by hibernation period in 2019~2021. 47
Fig. 1. Adult of the Luciola lateralis. A, Male; B, Female. 20
Fig. 2. Rearing and hibernation room of Luciola lateralis. A, Laboratory of indoor breeding room; B, Hibernation room. 21
Fig. 3. Rearing container. A, Rearing container designed to come up to land from water for to induce pupa and adult(Climbing box);... 22
Fig. 4. Collection of emerging adult of Luciola lateralis. 23
Fig. 5. Cage for investigation of egg period of Luciola lateralis. Cage (L72xW72xH100mm) filled with 1cm soil and oviposited... 24
Fig. 6. Cage for investigation of growth characteristics of Luciola lateralis. A, Square cage(L72 × W72 × H100mm); B, Round cage(100... 25
Fig. 7. Rearing and hibernation cage for larvae of Luciola lateralis. A, 50 larvae in square cage with 30mm of water without shelter; B,... 25
Fig. 8. Hibernation place for larvae of Luciola lateralis. 26
Fig. 9. Cage for comparison of oviposited numbers with 1cm soil for moisture retention. A, In 6㎠ moss; B. In 6㎠ nonwoven fabric. 27
Fig. 10. Types of rearingg cages an existing method. A, Round breeding cage(310 × H95mm); B, Square breeding cage(L460 ×... 29
Fig. 11. An existing method for larval rearing of Luciola laterlis. A, Feeding a clipped melanin snail; B, Removal of leftover food... 30
Fig. 12. Replenishment of water level due to evaporation during hibernation. 30
Fig. 13. Luciola lateralis larvae hibernated for 5-6 months. 31
Fig. 14. Investigation of survival rate for larvae of Luciola lateralis after hibernation. 31
Fig. 15. Placement of the Luciola lateralis larvae into the climbing box. 32
Fig. 16. Square breeding cage(L460 × W319 × H244mm) of automatic water change system. 95mm high with 30mm... 33
Fig. 17. Device for overflowing water. A, Hose(12/16mm); B, Hose elbow(16A); C, Bulkhead(15A); D, PVC valve... 33
Fig. 18. Installtion of the water overflow device in the square breeding cage. 34
Fig. 19. Filtering to prevent the larval overflow. PVC pipe(16A) with nylon filter(40, 100mesh) attached. 34
Fig. 20. Drain leading to a washbasin. A, Drainage made of PVC pipe (40A), t socket (40A), elbow socket (40A), cap (40A); B, Insert the hose from the overflow device into the drainage T socket; C,... 35
Fig. 21. Automatic water supply system. A, Connect the hose(6mm) and ball valve(6mm) to the water supply line of the washbasin; B, Connect the hose(6mm) to the solenoid valve(1/4) and then connect the... 36
Fig. 22. Overall automatic water change system view. 37
Fig. 23. Hibernation period for larvae of Luciola lateralis for 6, 12, and 18 months. 39
Fig. 24. Egg hatching process. A, 1day; B, 12days; C, 20days; D, 22days hatching. 40
Fig. 25. 1 to 6th instar larva of Luciola lateralis larva. A, 1st larva; B, 2nd larva; C, 3rd larva; D, 4th larva; E, 5th larva; F, 6th larva.[이미지참조] 42
Fig. 26. Comparison of moss and nonwoven for oviposited eggs. (A) Moss; (B) Nonwovenfabric. 43