표제지
목차
Ⅰ. Introduction 8
Ⅱ. Experimental 12
Ⅱ-1. 지역별 돈사 타액 수집 프로토콜 12
Ⅱ-2. 타액 수집 및 보관 방법 14
Ⅱ-3. 시약 및 장비 17
Ⅱ-4. 각 바이오마커의 ELISA 19
1) Sandwich ELISA 19
2) Competitive ELISA 24
3) HRP conjugation method 27
Ⅲ. Results and Discussion 30
Ⅲ-1. Stress Biomarker Calibration Curve. 30
Ⅲ-2. ELISA 결과그래프 33
1) 모돈의 수정 전과 후에 따른 스트레스 바이오마커 결과 33
2) 공조시설 유무에 따른 스트레스 바이오마커 결과 36
Ⅲ-3. T-test 결과 42
Ⅳ. Conclusion 46
Ⅴ. References 48
Abstract 55
국문요약 57
Figure 1. Places of collection of saliva. 13
Figure 2. Piglet saliva rope collection method. 15
Figure 3. Sow saliva rope collection method. 16
Figure 4. Absorbance measurement equipment. 18
Figure 5. Analysis method using sandwich ELISA. 20
Figure 6. Cortisol analysis method using competitive ELISA. 26
Figure 7. HRP conjugation. 28
Figure 8. TMB's color principle and chemical structure. 29
Figure 9. Calibration curves for the four biomarkers. 32
Figure 10. Modified stress results of the Handon Innovation Center. 35
Figure 11. Concentration of amylase in pig saliva in places (A) with and (B) without air conditioning facilities. 38
Figure 12. Concentration of IgA in pig saliva in places (A) with and (B) without air conditioning facilities. 39
Figure 13. Concentration of Cortisol in pig saliva in places (A) with and (B) without air conditioning facilities. 40
Figure 14. Concentration of CRP in pig saliva in places (A) with and (B) without air conditioning facilities. 41
Figure 15. Modified stress data analysis using T-test. 43
Figure 16. Air conditioning facility comparison data analysis using T-test. 45