표제지
목차
국문초록 8
I. Introduction 10
II. Material & Method 13
1. Mice 13
2. Reagents 13
3. B cell isolation and CD40LB co-culture 14
4. RNA extraction and qRT-PCR 15
5. Western blotting 16
6. Flow Cytometry 16
7. Influenza A virus infection 16
8. ELISA (enzyme-linked immunosorbent assay) 17
9. Virus plaque assay 17
10. Statistical analysis 18
III. Results 19
1. In vitro co-cultivation system to differentiate naive B cells into GC B cells and antibody-producing plasmablast 19
2. Inhibition of mTOR signaling enhances Ig class switching of activated B cell 23
3. Negatively regulates Ig class switching of plasmablasts in the context of PI3K-Pten signaling pathway 26
4. Suppression of mTOR signaling by rapa and torin-II partially restored Ig class switching of activated B cells lacking PTEN 30
5. mTORC2 inactivation restores Ab class switching of Pten-depleted B cells in response to influenza infection 33
6. Antibody response of B cells requires a modulation of the PI3K-signaling through Pten-mTOR axis. 36
IV. Discussion 40
V. References 45
Abstract 49
Table 1. List of quantitative Real-timePCR primer 6
Figure 1. In vitro co-cultivation system to generate iGC B cells and iPB cells 22
Figure 2. Inhibition of mTOR signaling enhances Ig class switching of activated B cell 25
Figure 3. Negatively regulates Ig class switching of plasmablasts in the context of PI3K-Pten signaling pathway 29
Figure 4. Suppression of mTOR signaling by rapa and torin-II partially restored Ig class switching of activated B cells lacking PTEN 32
Figure 5. mTORC2 inactivation restores Ab class switching of Pten-depleted B cells in response to influenza infection 35
Figure 6. DEG (Differentially Expressed Genes) associated with antibody class switching were inspectulated and identified in RNA-seq analysis 39