Title Page
Contents
ABBREVIATIONS 9
국문요약 11
I. INTRODUCTION 13
II. MATERIALS AND METHODS 21
1. Materials 21
2. Bacterial culture 21
3. Agar dilution test to determine MIC 21
4. Broth dilution test to determine MIC 22
5. Reverse transcriptase-polymerase chain reaction (RT-PCR) 22
6. Western blot 26
7. Urease activity test 27
8. Statistical analysis 27
III. RESULTS 28
1. The inhibitory effect of saucerneol D on the growth of H. pylori 28
2. Effects of saucerneol D on the expression of replication and transcription genes in H. pylori 31
3. Effects of saucerneol D on the expression of major virulence factors in H. pylori 34
4. Downregulation of urease in H. pylori treated with saucerneol D 40
5. Effect of flagella and adhesion in H. pylori treated with saucerneol D 44
IV. DISCUSSION 47
V. REFERENCE 51
Abstract 62
Table 1. List of primer sequences and PCR conditions for RT-PCR. 24
Figure 1. Structure of type IV secretion system in H. pylori. 16
Figure 2. Structure of flagella in H. pylori. 18
Figure 3. Chemical structure of saucerneol D. 20
Figure 4. Determination of the MIC of saucerneol D against H. pylori H. pylori by agar dilution. 29
Figure 5. Determination of the MIC of saucerneol D against H. pylori H. pylori by broth dilution. 30
Figure 6. Expression of replication-related genes in H. pylori treated with saucerneol D. 32
Figure 7. Expression of transcription-related genes in H. pylori treated with saucerneol D. 33
Figure 8. Expression of cagA and vacA in H. pylori treated with saucerneol D. 35
Figure 9. Expression of CagA and VacA in H. pylori treated with saucerneol D. 36
Figure 10. Expression of T4SS genes in H. pylori treated with saucerneol D. 37
Figure 11. Expression of secA in H. pylori treated with saucerneol D. 38
Figure 12. Expression of SecA in H. pylori treated with saucerneol D. 39
Figure 13. Expresstion of urease subunots in H. pylori treated with saucerneol D. 41
Figure 14. Expresstion of urease subunits in H. pylori treated with saucerneol D. 42
Figure 15. Downregulation of urease activity in H. pylori treated with saucerneol D. 43
Figure 16. Expression of flagella genes in H. pylori treated with saucerneol D. 45
Figure 17. Expression of adherence genes in H. pylori treated with saucerneol D. 46