표제지
목차
국문 요약 10
I. 서론 12
1. 연구배경 12
2. 가시오갈피의 성상 15
3. 가시오갈피의 한방효능 17
4. 가시오갈피의 성분과 효능 및 약리작용 18
5. 오갈피의 페놀성 성분 22
6. 연구필요성 및 목적 23
II. 실험재료 및 성분분석 25
1. 실험재료 25
가. 한국산 가시오갈피 줄기 채집 및 제조 25
나. 한국산 가시오갈피 잎 채집 및 제조 25
2. 시료 엑스 조제 28
가. 한국산 가시오갈피 줄기 70% Ethyl alcohol 엑스 조제 28
나. 한국산 가시오갈피 잎 70% Ethyl alcohol 엑스 조제 28
3. 한국산 가시오갈피 성분분석 29
III. 결과 및 고찰 30
1. 한국산 가시오갈피 줄기 70% Ethyl alcohol 추출물 30
2. 한국산 가시오갈피 잎 70% Ethyl alcohol 추출물 32
IV. 결론 58
참고문헌 59
Abstract 67
Table 1. HPLC gradient conditions of 70% Ethyl alcohol extraction conditions of Korean Acanthopanax senticosus stem 34
Table 2. Extraction yield of Korean Acanthopanax senticosus stem 35
Table 3. The phenolic compounds according to 70% Ethyl alcohol extraction conditions of Korean Acanthopanax senticosus stem 36
Table 4. HPLC gradient conditions of 70% Ethyl alcohol extraction conditions of Korean Acanthopanax senticosus leaf 47
Table 5. Extraction yield of Korean Acanthopanax senticosus leaf 48
Table 6. The phenolic compounds according to 70% Ethyl alcohol extraction conditions of Korean Acanthopanax senticosus leaf 49
Fig. 1. Formulae of the phenolic constituents of Acanthopanacis Caulis 22
Fig. 2. The photograph of Korean Acanthopanax senticosus stem 26
Fig. 3. The photograph of Korean Acanthopanax senticosus leaf 27
Fig. 4. Comparison of contents of total phenolic compounds according to 70% Ethyl alcohol extraction process of... 37
Fig. 5. Comparison of contents of eleutheroside B according to 70% Ethyl alcohol extraction process of Korean Acanth-... 38
Fig. 6. Comparison of contents of chlorogenic acid according to 70% Ethyl alcohol extraction process of Korean Acantho-... 39
Fig. 7. Comparison of contents of syringaresinol according to 70% Ethyl alcohol extraction process of Korean... 40
Fig. 8. HPLC profiles of mixed-standards for 70% Ethyl alcohol extracts conditions of Korean Acanthopanax senticosus stem 41
Fig. 9. HPLC profiles of phenolic compounds according to 70% Ethyl alcohol extraction(EAS-1) conditions of Korean... 42
Fig. 10. HPLC profiles of phenolic compounds according to 70% Ethyl alcohol extraction(EAS-2) conditions of Korean... 43
Fig. 11. HPLC profiles of phenolic compounds according to 70% Ethyl alcohol extraction(EAS-3) conditions of Korean... 44
Fig. 12. HPLC profiles of phenolic compounds according to 70% Ethyl alcohol extraction (EAS-4) conditions of Korean... 45
Fig. 13. HPLC profiles of phenolic compounds according to 70% Ethyl alcohol extraction(EAS-5) conditions of Korean... 46
Fig. 14. Comparison of contents of total phenolic compounds according to 70% Ethyl alcohol extraction process of... 50
Fig. 15. Comparison of contents of eleutheroside B according to 70% Ethyl alcohol extraction process of Korean Acanth-... 51
Fig. 16. Comparison of contents of chlorogenic acid according to 70% Ethyl alcohol extraction process of Korean Acanth-... 52
Fig. 17. Comparison of contents of Syringresinol according to 70% Ethyl alcohol extraction process of Korean... 53
Fig. 18. HPLC profiles of mixed-standards for 70% Ethyl alcohol extracts conditions of Korean Acanthopanax... 54
Fig. 19. HPLC profiles of phenolic compounds according to 70% Ethyl alcohol extraction(EAL-1) conditions of Korean... 55
Fig. 20. HPLC profiles of phenolic compounds according to 70% Ethyl alcohol extraction(EAL-2) conditions of Korean... 56
Fig. 21. HPLC profiles of phenolic compounds according to 70% Ethyl alcohol extraction(EAL-3) conditions of Korean... 57