Title Page
Contents
초록 7
ABSTRACT 8
INTRODUCTION 9
MATERIALS AND METHODS 12
Cell culture and cell lines 12
Antibodies 12
Western Blotting 12
In vivo ISGylation assay 13
Real-time PCR and primers 13
RESULTS 15
CtBP1 is a target of ISGylation 15
USP18 and EFP regulate ISGylation of CtBP1 15
ISGylation of CtBP1 enhances interaction with its binding partner 16
ISGylation of CtBP1 represses expression of target gene 17
DISCUSSION 27
Reference 29
Figure 1. CtBP1 is a target of ISGylation 18
Figure 2. USP18 and EFP regulate ISGylation of CtBP1 20
Figure 3. ISGylation of CtBP1 enhances interaction with its binding partner 22
Figure 4. ISGylation of CtBP1 represses expression of target gene 25