Title Page
Abstract
Contents
List of Abbreviations 8
1. Introduction 11
2. Materials and Methods 14
2.1. Materials 14
2.2. Synthesis of gold nanoparticles 15
2.3. AuNP conjugation with norovirus binding peptides 16
2.4. Preparation of lateral flow test strips 20
2.5. Lateral flow strip assay of norovirus virus-like particles (VLPs) 22
3. Results and Discussion 23
3.1. Selection of peptide self-assembled monolayer on gold nanoparticle 23
3.2. Characterization and optimization of NoroBP binding AuNPs 24
3.3. Preparation of the test strip 26
3.4. Lateral flow assay test 28
4. Conclusions 30
5. References 30
Fig.1. Synthesis of AuNPs. A) AuNP snapshot B) UV-vis spectrum and C) TEM images of AuNPs. 17
Fig.2. Gold aggregation test(GAT). A. Pure 40nm AuNPs and AuNPs/NoroBP were prepared. Then, 100 μL of 10% NaCl solution were added for 5 min under... 18
Fig.3. Characterization of 40 nm AuNPs and AuNP/NoroBP conjugates: UV-vis spectrum of AuNP solution. And different amount of NoroBP... 19
Fig.4. Schematic figure of AuNP/NoroBP lateral flow assay 21
Fig.5. Schematic figure of Norovirus binding peptides 24
Fig.6. Blocking the membrane with different capping agent. A. Non-specific binding test with D.W. B: Color intensity test with sample (1: 2% BSA, 2: 1%... 27
Fig.7. LFA for norovirus detection using 1) DI water 2) Norovirus-VLP(107 particles/ml)[이미지참조] 29