표제지
목차
국문요약 9
I. Introduction 11
II. Materials and Methods 15
1. Materials 15
2. Cell culture 15
3. Cytotoxicity assay 15
4. Real-time RT-PCR 16
5. Western blot 19
6. TEER (Transepithelial Electrical Resistance) assay 21
7. Statistical processing 21
III. Results and Discussion 22
1. Cellular safety and effects of statin drugs 22
2. Inhibition of keratinocyte differentiation-associated inflammatory cytokines by mevastatin 25
3. Mevastatin inhibits the expression of immune-inducing chemokines in psoriatic skin 28
4. Inhibition of keratin expression by mevastatin in TNF-α-induced HaCaT cells 30
5. Inhibition of phosphorylation of IκB protein by mevastatin in TNF-α-induced HaCaT cells 32
6. Inhibition of phosphorylation of MAPKs (ERK1/2, JNK, p38) proteins by mevastatin in TNF-α-induced HaCaT cells 34
7. Inhibition of phosphorylation of STAT3 proteins by mevastatin in TNF-α-induced HaCaT cells 36
8. Effects of mevastatin on improving skin barrier 38
9. Inhibition of IL-6 and CCL20 mRNA expression by mevastatin in IL-17A-induced HaCaT cells 40
10. Inhibition of STAT3 protein expression by mevastatin in IL-17A-induced HaCaT cells 42
IV. Conclusion 45
V. References 48
Abstract 55
Appendices 57
Appendix 1. 2019. 05. 17. 대한화장품학회 학술대회 57
Appendix 2. 2019. 10. 11. 한국생물공학회 학술대회 58
Appendix 3. Journal of Biotechnology and Bioprocess Engineering (BBE) 게시 논문 59
Table 1. Gene name and assay ID number in real-time PCR analysis 18
Table 2. Antibody list used in experiments 20
Fig. 1. (A) Structure of fungal-derived statins (mevastatin, lovastatin,... 23
Fig. 2. Mevastatin inhibits the expression of cytokines involved in the... 27
Fig. 3. Mevastatin inhibits the expression of chemokines activated by TNF-... 29
Fig. 4. Mevastatin inhibits the expression of keratin 5/14 and 6A/16... 31
Fig. 5. Mevastatin regulates IκB phosphorylation in TNF-α-induced HaCaT... 33
Fig. 6. Mevastatin inhibits phosphorylation of MAPK (ERK1/2, JNK, p38)... 35
Fig. 7. Mevastatin inhibits phosphorylation of the STAT3 protein.... 37
Fig. 8. Mevastatin improves the deteriorated skin barrier. TNF-α worsened... 39
Fig. 9. Mevastatin inhibits the expression of IL-6 and CCL20 in... 41
Fig. 10. Mevastatin inhibits the expression of STAT3 in IL-17A-induced... 43
Fig. 11. In keratinocytes, mevastatin exhibits anti-inflammatory and... 44