This study aims to test whether and to what extent national culture affects the readiness of Business-to-Consumer E-commerce (B2C E-commerce) across the world. The study regresses two sets of data at national level: (1) the scores of six dimensions of Hofstede's national culture framework, power distance, uncertainty avoidance, individualism, masculinity, long-term orientation and indulgence; (2) United Nations Conference on Trade and Development (UTCTAD) B2C E-commerce index value. Multi-rounds of linear regression of a sample of 62-83 countries, which is more than twice size of previous similar research (Lim et al., 2004), are conducted in this study. Several control variables at national level are included in our regression models. The results show that two cultural dimensions of individualism and uncertainty avoidance have consistent effects on the readiness of B2C E-commerce in each step of testing. Findings show that countries with higher individualism ratings tend to have higher B2C E-commerce index value (the higher readiness level of B2C E-commerce), which verifies the results in literature. It also reveals countries with higher long-term orientation tend to have higher B2C E-commerce index value. Long-term oriented societies tend to focus on the future (Hofstede, 2001). B2C E-commerce adoption will enhance the economic competitiveness of enterprises as well as the whole country in the future. At individual level, people of a high long-term orientation culture allow them to take risk to try new things, which is beneficial for the readiness of B2C E-commerce. Therefore, long-term orientation is positively related with the readiness of B2C E-commerce, which fills the gap on research of this respect and creates the direct link between long-term orientation and B2C E-commerce. The other four dimensions don't show consistent impacts on B2C E-commerce in each step of testing but significant results in some step separately, which is also enlightening for future research. Then, the study discusses the implications of these findings for B2C E-commerce adopting strategies. The researcher then calls for designing relative policies with full consideration on national culture to promote the adoption of B2C E-commerce. In addition, the researcher identifies the limitations of the study and propose recommendations for future research. This study is the first one to use UNCTAD data on B2C E-commerce to explore the relationships between all six dimensions of Hofstede's national culture and the readiness of B2C E-commerce and adds to the knowledge of literature in this research field.