Title Page
Contents
Abstract 9
I. INTRODUCTION 11
II. MATERIALS AND METHODS 14
1. Materials 14
2. Liposome preparation 15
3. Characterization of liposomes 15
3.1. Particle size and surface charge of liposomes 15
3.2. Encapsulation efficiency and contents of drug in liposomes 16
4. Ultrasonic spray coating 16
5. Drug release study 17
6. Cell culture 17
7. Cell viability assay 17
8. Cellular uptake of liposomes 18
9. Surface analysis of coated layer 18
10. Functionality of PVP layer 19
11. Protein adsorption 19
12. Live/dead assay 20
13. Inflammation test 20
14. Ex vivo test 20
15. Statistical analysis 21
III. RESULTS 23
1.1. Cationic liposome characterization 23
1.2. In vitro and ex vivo test (Drug-loaded liposome transfer to cell and tissue) 27
2.1. HSPC liposome characterization 30
2.2. Release test 33
2.3. In vitro study 35
2.4. Analysis of coated layer 37
2.5. Functionality of PVP coated layer 42
2.6. Cell viability test 45
2.7. Inflammation test 48
2.8. Protein adsorption 50
2.9. Ex vivo test 52
IV. DISCUSSION 54
V. CONCLUSION 57
REFERENCES 58
ABSTRACT IN KOREAN 63
Table 1.1. Zeta potential changes depending on the ratio of cationic lipid 24
Table 1.2. Drug transfer rate and the amount of remaining drug ex vivo study 29
Table 2.1. Characterization of XPS atomic concentration and… 41
Table 2.2. Transfer rate of drug-loaded liposome. 53
Figure 1.1. Characterization of cationic liposome and drug-loaded... 26
Figure 1.2. In vitro and ex vivo study with drug-loaded cationic liposome. 28
Figure 2.1. Optimization for drug encapsulation efficiency. 31
Figure 2.2. Characterization of liposome. 32
Figure 2.3. The amount of coated drug onto Nylon tubes. 34
Figure 2.4. In vitro study using liposome and drug-loaded liposome. 36
Figure 2.5. Characterization of PVP, PVP/liposome, and... 40
Figure 2.6. Functional analysis of PVP and drug-loaded liposome... 44
Figure 2.7. Cell viability test. 47
Figure 2.8. Inflammation test by ELISA. 49
Figure 2.9. Protein adsorption test. 51
Figure 2.10. Ex vivo study using Nile red labeled-liposome. 53
Scheme 1. Schematics of multilayer functionalized drug eluting balloon. 22