Title Page
Contents
Abstract 7
I. INTRODUCTION 9
II. MATERIALS AND METHODS 12
1. Human iPSC culture 12
2. MSN differentiation 12
3. Phenotype induction(BDNF,MG132) & HDAC6 inhibitor treatment 13
4. Immunocytochemistry 13
5. image quantification 14
6. Western blot 14
7. Statistical analysis 15
8. Cell information 15
III. RESULTS 16
Part.1. Medium spiny neuron(MSN) differentiation and HD pathology characterization derived HD patients iPSC 16
Part.2. medium spiny neuron derived CTL and HD iPSC was weakly inhibit Proteasome activity by MG132 19
Part.3. medium spiny neuron derived CTL and HD iPSC up-regulation UB by high BDNF 24
IV. DISCUSSION 27
REFERENCES 30
ABSTRACT IN KOREAN 34
Fig.1. MSN differentiation and pathology characterization in iPSC... 18
Fig.2. induced phenotype and HDAC6 inhibitor effect using MG132 and... 23
Fig.3. induced phenotype and HDAC6 inhibitor effect using high... 26
Fig.4. Schematic diagram of UPS affected by rejuvenation, MG132, high... 29