Title Page
Contents
Abstract 8
I. INTRODUCTION 10
II. MATERIALS AND METHODS 12
II.1. Materials 12
II.2. Synthesis of RA-MH 12
II.3. Characterization of RA-MH 12
II.4. pH titration of RA-MH 13
II.5. Preparation of RA-MH-loaded PLGA microspheres (RA-MH/PLGA microspheres) 13
II.6. Morphological analysis of RA-MH/PLGA microspheres 14
II.7. pH titration of RA-MH/PLGA microspheres 14
II.8. In vitro cell culture 14
II.9. In vitro biocompatibility test 14
II.10. Measurement of pro-inflammatory cytokines expression 15
II.11. Statistical analysis 15
III. Results and Discussion 16
III.1. Synthesis and characterization of RA-MH 16
III.2. Preparation and characterization of RA-MH/PLGA microspheres 17
III.3. In vitro biocompatibility of RA-MH/PLGA microspheres 18
III.4. In vitro anti-inflammatory effect of RA-MH/PLGA microspheres 19
IV. CONCLUSION 20
REFERENCES 21
ABSTRACT IN KOREAN 30
Table 1. Characterization of MH/PLGA and RA-MH/PLGA microspheres 25
Figure 1. Schematic illustration of (a) Synthetic route for ricinoleic acid-capped magnesium... 26
Figure 2. Characterization of RA-MH. (a) Digital photo of large-scale synthesis of RA-MH, (b)... 27
Figure 3. Characterization of RA-MH-encapsulated PLGA microspheres (RA-MH/PLGA ms). (a)... 28
Figure 4. In vitro biocompatibility and anti-inflammatory effect of degradation product of... 29