Title Page
Contents
Abstract 8
I. INTRODUCTION 10
II. MATERIALS AND METHODS 13
1. Cell lines 13
2. Hypoxia 14
3. Flow cytometry 15
4. Cytotoxicity assay 16
5. TCGA data 17
6. Statistical analysis 18
III. RESULTS 19
1. The expression level of ADAM9 affects the prognosis of patients with glioma patients including GBM 19
2. Hypoxia marker HIF-1α and its responsive protein GLUT-1 were increased under hypoxic conditions 22
3. Proliferation of U343 and U251 cell lines was reduced under hypoxic conditions. 25
4. ADAM9 was significantly increased hypoxia than normoxia. 27
5. MICA was significantly increased normoxia than hypoxia. 29
6. NK-mediated cytotoxicity was reduced in hypoxia. 31
IV. DISCUSSION 35
V. CONCLUSION 37
REFERENCES 38
ABSTRACT IN KOREAN 44
Figure 1. GBM patients with high ADAM9 expression have a low... 21
Figure 2. Under hypoxic conditions, HIF-1α and GLUT-1 in U343 and... 24
Figure 3. Differences in proliferation of GBM cell lines between... 26
Figure 4. ADAM9 was increased under hypoxic conditions. 28
Figure 5. MICA decreased under hypoxic conditions. 30
Figure 6. NK-92 mediated cytotoxicity against U343, U251 cell lines... 34