표제지
목차
I. 서론 11
1. 연구의 배경 11
2. 연구의 필요성 13
3. 연구의 목적 14
II. 재료 및 시약 15
1. 실험재료 및 시약 15
III. 방법 16
1. 이화학실험 16
1.1. 일반성분분석 16
1.2. 향기성분 분석 18
2. 극성에 따른 용매추출 19
2.1. 용매추출 19
2.2. GC/MSD 21
2.3. GC-olfactometry 21
2.4. E-nose 21
3. 동물실험 22
3.1. 동물 사육 방법 22
3.2. 향기 흡입 방법 22
3.3. 구속 스트레스 모델 제작 24
3.4. 수면 스트레스 모델 제작 24
3.5. 혈청 및 장기적출 28
3.6. 혈청 및 장기실험 28
4. 통계처리 36
5. 윤리위원회 승인 36
IV. 결과 및 고찰 37
1. 이화학실험 37
1.1. 일반성분 분석 37
1.2. 향기성분 분석 40
2. 극성에 따른 용매 추출 44
2.1. GC/MSD 44
2.2. GC-olfactometry 48
2.3. E-nose 50
3. 동물실험 54
3.1. 구속 스트레스 54
3.2. 수면 스트레스 66
V. 인용문헌 79
ABSTRACT 85
국문초록 88
Table 1. A week plan of animal experiments 25
Table 2. Proximate compositions in Osmanthus fragrans var. aurantiacu 38
Table 3. Change of constituent amino acid and free amino acid profiles in... 39
Table 4. Volatile compounds of Osmanthus fragrans var. aurantiacu 41
Table 5. GC-olfactometry data of Osmanthus fragrans var. aurantiacu 43
Table 6. Changes of volatile compounds according to extraction solution of Osmanthus fragrans var. aurantiacu 46
Table 7. Changes of volatile compounds according to extraction solution of Osmanthus fragrans var. aurantiacu by... 49
Table 8. Changes of volatile compounds according to extraction solution of Osmanthus fragrans var. aurantiacu by E-nose(MXT-5) 51
Table 9. Changes of volatile compounds according to extraction solution of Osmanthus fragrans var. aurantiacu by E-nose(MXT-1701) 52
Table 10. Sensory description of volatile compounds in Osmanthus fragrans var.... 53
Table 11. Food intake amounts in restraint groups 56
Table 12. Changes of weight in restraint groups 57
Table 13. Changes of length in restraint groups 58
Table 14. Changes of waist in restraint groups 59
Table 15. Weight gain(g), length gain(cm), waist gain(cm) in restraint groups 60
Table 16. FER of inhalation restraint groups 61
Table 17. Organ and tissue weights of restration groups 63
Table 18. Effects of cholesterol on restration groups 65
Table 19. Effects of hormone on restration groups 65
Table 20. Food intake amount in sleep deprivation groups 68
Table 21. Changes of weight in sleep deprivation groups 69
Table 22. Changes of length in sleep deprivation groups 70
Table 23. Changes of waist in sleep deprivation groups 71
Table 24. Weight gain(g), length gain(cm), waist gain(cm) in sleep deprivation groups 72
Table 25. FER of sleep deprivation groups 73
Table 26. Organ and tissue weights of sleep deprivation groups 75
Table 27. Effects of cholesterol on sleep deprivation groups 78
Table 28. Effects of hormone on sleep deprivation groups 78
Figure 1. Extracted Osmanthus fragrans car. aurantiacuin in 4. solvents with... 20
Figure 2. Inhalation of the volatile compounds in Osmanthus fragrans car.... 23
Figure 3. Restraint model. 26
Figure 4. Sleep deprivation model. 27
Figure 5. Food intake amounts in restraint groups. 56
Figure 6. Changes of weight in restraint groups. 57
Figure 7. Changes of length in restraint groups. 58
Figure 8. Changes of waist in restraint groups. 59
Figure 9. Weight gain(g), length gain(cm), waist gain(cm) in restraint groups. 60
Figure 10. FER of inhalation restraint groups. 61
Figure 11. Food intake amounts in sleep deprivation groups. 68
Figure 12. Changes of weight in sleep deprivation groups. 69
Figure 13. Changes of length in sleep deprivation groups. 70
Figure 14. Changes of waist in sleep deprivation groups. 71
Figure 15. Weight gain(g), length gain(cm), waist gain(cm) in sleep deprivation groups. 72
Figure 16. FER of sleep deprivation groups. 73