표제지
목차
I. 서론 8
II. 재료 및 방법 13
1. Isoquercitrin 생산 박테리아 분리 및 종 식별 13
2. 박테리아 동정 13
3. 형질전환 competent cell 제조 14
4. 생물전환 15
5. HPLC분석 16
III. 결과 및 고찰 17
1. 차나무 식재지의 배양 가능한 박테리아의 수 17
2. Isoquercitrin 생산 박테리아 분리 17
3. 16S rRNA유전자를 이용한 Isoquercitrin 생산 박테리아 동정 18
4. 반응성 검증 21
5. 배지에 따른 Bacillus sp. CSQ10의 반응성 검증 25
6. Isoquercitrin 생산 최적화 29
7. Quercetin-3-O-(6-acetyl glucoside) 생산 최적화 33
IV. 결론 38
참고문헌 39
국문초록 44
ABSTRACT 46
Table 1. Characteristics of isoquercitrin 11
Figure 1. Classification of flavonoids. Flavonoids are classified into six groups, including... 9
Figure 2. Nucleotide sequence of 16S rRNA gene from Bacillus sp. CSQ10 isolated from... 19
Figure 3. A neighbor-joining phylogenetic tree based on the 16S rRNA gene of Bacillus... 20
Figure 4. HPLC analysis of reaction product by biotransformation of Bacillus sp CSQ10... 23
Figure 5. MS spectra of reaction product 1 in positive ion mode(A) and negative ion mode(B) 24
Figure 6. Effect of biotransformation medium. The cells were cultured at 30℃ for over... 26
Figure 7. HPLC analysis of reaction product by biotransformation of Bacillus sp. CSQ10... 27
Figure 8. MS spectra of reaction product 2 in positive ion mode 28
Figure 9. Effect of biotransformation temperature on isoquercitrin production by Bacillus... 30
Figure 10. Determination of optimum cell concentration for isoquercitrin production from... 31
Figure 11. Determination of optimum quercetin feed concentration for isoquercitrin... 31
Figure 12. Production of isoquercitrin using Bacillus sp. CSQ10 and 600 μM quercetin.... 32
Figure 13. Effect of biotransformation temperature on quercetin-3-O-(6-acetyl glucoside)... 34
Figure 14. Determination of optimum cell concentration for quercetin-3-O-(6-acetyl... 35
Figure 15. Determination of optimum quercetin feed concentration for... 36
Figure 16. Production of quercetin-3-O-(6-acetyl glucoside) using Bacillus sp. CSQ10 and 600... 37