표제지
목차
국문 초록 13
I. 서론 15
II. 재료 및 방법 17
1. 재료 17
1) 시료 17
2) 시약 18
3) 기기 19
2. 방법 19
1) 세포배양 19
2) Nitric Oxide (NO) 생성량 측정 19
3) 동물 및 사료 20
4) 대장염 유발 및 시료처리 20
5) 대장 조직 분리 21
6) 유전자 발현량 측정 21
7) 단백질 활성화 측정 23
8) 조직염색 및 관찰 24
3. 통계처리 24
III. 실험 결과 25
1. In vitro 실험 결과 25
1) NO 생성량 25
2) 유전자 발현량 27
3) 단백질 활성화 47
2. In vivo 실험 결과 61
1) 대장 조직 길이 61
2) 조직학적 분석 63
3) 유전자 발현량 65
4) 단백질 활성화 85
IV. 고찰 93
V. 결론 102
참고문헌 104
ABSTRACT 113
Table 1. The Amount and Composition of Hwanggeum-tang (HGT)... 17
Table 2. The Sequences of Primers 23
Table 3. Effect of HGT and GST on NO Level in RAW 264.7 cells 25
Table 4. Effect of HGT and GST on PTGS2 mRNA Expression Level... 27
Table 5. Effect of HGT and GST on NOS2 mRNA Expression Level... 29
Table 6. Effect of HGT and GST on IL1B mRNA Expression Level in... 31
Table 7. Effect of HGT and GST on IL6 mRNA Expression Level in... 33
Table 8. Effect of HGT and GST on TNFA mRNA Expression Level... 35
Table 9. Effect of HGT and GST on IL10 mRNA Expression Level in... 37
Table 10. Effect of HGT and GST on HPGD mRNA Expression Level... 39
Table 11. Effect of HGT and GST on NQO1 mRNA Expression Level... 41
Table 12. Effect of HGT and GST on HO1 mRNA Expression Level in... 43
Table 13. Effect of HGT and GST on NFE2L2 mRNA Expression... 45
Table 14. Effect of HGT and GST on COX-2 Protein Expression Level... 47
Table 15. Effect of HGT and GST on STAT3 protein phosphorylation... 49
Table 16. Effect of HGT and GST on ERK protein phosphorylation in... 51
Table 17. Effect of HGT and GST on JNK protein phosphorylation in... 53
Table 18. Effect of HGT and GST on P38 protein phosphorylation in... 55
Table 19. Effect of HGT and GST on HO-1 Protein Expression Level... 57
Table 20. Effect of HGT and GST on NRF2 Protein Expression Level... 59
Table 21. Effect of HGT and GST on Colon Length in DSS-induced... 61
Table 22. Effect of HGT and GST on PTGS2 mRNA Expression Level... 65
Table 23. Effect of HGT and GST on NOS2 mRNA Expression Level... 67
Table 24. Effect of HGT and GST on IL1B mRNA Expression Level... 69
Table 25. Effect of HGT and GST on IL6 mRNA Expression Level in... 71
Table 26. Effect of HGT and GST on TNFA mRNA Expression Level in... 73
Table 27. Effect of HGT and GST on IL10 mRNA Expression Level in... 75
Table 28. Effect of HGT and GST on HPGD mRNA Expression Level... 77
Table 29. Effect of HGT and GST on NQO1 mRNA Expression Level... 79
Table 30. Effect of HGT and GST on HO1 mRNA Expression Level in... 81
Table 31. Effect of HGT and GST on NFE2L2 mRNA Expression Level... 83
Table 32. Effect of HGT and GST on COX-2 Protein Expression Level... 85
Table 33. Effect of HGT and GST on STAT3 protein phosphorylation in... 87
Table 34. Effect of HGT and GST on HO-1 Protein Expression Level... 89
Table 35. Effect of HGT and GST on NRF2 Protein Expression Level... 91
Fig. 1. Effect of HGT and GST on NO level in RAW 264.7... 26
Fig. 2. Effect of HGT and GST on PTGS2 mRNA expression... 28
Fig. 3. Effect of HGT and GST on NOS2 mRNA expression... 30
Fig. 4. Effect of HGT and GST on IL1B mRNA expression... 32
Fig. 5. Effect of HGT and GST on IL6 mRNA expression... 34
Fig. 6. Effect of HGT and GST on TNFA mRNA expression... 36
Fig. 7. Effect of HGT and GST on IL10 mRNA expression... 38
Fig. 8. Effect of HGT and GST on HPGD mRNA expression... 40
Fig. 9. Effect of HGT and GST on NQO1 mRNA expression... 42
Fig. 10. Effect of HGT and GST on HO1 mRNA expression... 44
Fig. 11. Effect of HGT and GST on NFE2L2 mRNA... 46
Fig. 12. Effect of HGT and GST on COX-2 protein expression... 48
Fig. 13. Effect of HGT and GST on STAT3 protein... 50
Fig. 14. Effect of HGT and GST on ERK protein... 52
Fig. 15. Effect of HGT and GST on JNK protein... 54
Fig. 16. Effect of HGT and GST on P38 protein... 56
Fig. 17. Effect of HGT and GST on HO-1 protein expression level... 58
Fig. 18. Effect of HGT and GST on NRF2 protein expression... 60
Fig. 19. Effect of HGT and GST on colon length in... 62
Fig. 20. Representative colon sections stained with hematoxylin... 64
Fig. 21. Effect of HGT and GST on PTGS2 mRNA expression... 66
Fig. 22. Effect of HGT and GST on NOS2 mRNA expression... 68
Fig. 23. Effect of HGT and GST on IL1B mRNA expression... 70
Fig. 24. Effect of HGT and GST on IL6 mRNA expression... 72
Fig. 25. Effect of HGT and GST on TNFA mRNA expression... 74
Fig. 26. Effect of HGT and GST on IL10 mRNA expression... 76
Fig. 27. Effect of HGT and GST on HPGD mRNA expression... 78
Fig. 28. Effect of HGT and GST on NQO1 mRNA expression... 80
Fig. 29. Effect of HGT and GST on HO1 mRNA expression... 82
Fig. 30. Effect of HGT and GST on NFE2L2 mRNA... 84
Fig. 31. Effect of HGT and GST on COX-2 protein expression... 86
Fig. 32. Effect of HGT and GST on STAT3 protein... 88
Fig. 33. Effect of HGT and GST on HO-1 protein expression... 90
Fig. 34. Effect of HGT and GST on NRF2 protein expression... 92