OBJECTIVES: This experimental study was designed to investigate the effects of Hwanggwaru-whan and Chibiinseubdam-bang on the anti-obesity effect in obese rats induced by the high-fat diet.
METHODS: The white rats were divided into five groups. The normal group that did not cause obesity (Normal), Control group that caused obesity through diet and did not receive medication (Control), Positive Control group that induced obesity through diet and administered Xenical 40 mg/kg (PC(Xenical)), Experimental group inducing obesity and administering Hwanggwaru-whan 250 mg/kg (HGRH), Experimental group inducing obesity and administering Chibiinseubdam-bang 250 mg/kg (CBSB). Indices of obesity, such as body weight and feed intake were measured for six weeks. Total cholesterol, TG, HDL-cholesterol, LDL-cholesterol, free fatty acid, phospholipid, GOT, GPT. RBC, WBC, Plt, Hb were measured after six weeks. Total RNA was isolated, and adiponectin, PPAR-γ, and leptin were measured using PC-RCT. In order to observe the intracellular fat accumulation of HGRH and CBSB, 3T3-L1 adipocytes were distributed, and intracellular TGs were observed through Oil red O staining and TG kit.
RESULT: Both HGRH and CBSB lost weight compared to the Control group. At week 6, a statistically significant decrease was observed in PC(Xenical) and HGRH. There was a lot of difference between the intakes of the high-fat feed and the normal feed, but no significant difference was observed between the experimental groups that consumed the high-fat feed. In PC(Xenical), HGRH, and CBSB, TG, total cholesterol, LDL cholesterol, and free fatty acid all showed statistically significant decreases compared to Control. As a result of measuring HDL-cholesterol, PC(Xenical), HGRH, and CBSB all tended to decrease compared to Control, but there was no statistical significance. As a result of measuring the phospholipid content, PC(Xenical), HGRH, and CBSB all tended to decrease compared to Control, and only CBSB was statistically significant. In both HGRH and CBSB, TG accumulated in 3T3-L1 cells was stained with Oil Red O solution and observed by concentration. As a result of observing the content of TG in cells accumulated during fat metabolism using the TG kit, HGRH showed a statistically significant decrease, and CBSB tended to decrease, but not statistically significant. As a result of observing the effect on the expression of adiponectin, PPAR-γ, and leptin using RT-PCR, a significant increase in HGRH and CBSB was observed in adiponectin compared to Control. In the PPAR-γ category, a significant increase was observed in CBSB, and in leptin, a significant decrease was observed in PC(Xenical), HGRH, and CBSB compared to Control. As a result of measuring GOT, GPT, and CBC, there was no significant difference between groups.
CONCLUSION: As a result of examining the anti-obesity effects of Hwanggwaru-whan(HGRH) and Chibiinseubdam-bang(CBSB), it was found that the serum test of high-fat diet-induced white rats reduced obesity indicators and affects the expression of factors involved in the adipocyte differentiation process.