표제지
목차
요약 9
I. 서론 11
II. 실험방법 13
1. 실험 재료 13
1) 상황버섯 시료 준비 13
2) 상황버섯 초임계 추출 13
3) 상황버섯 단백질 추출 14
4) 상황버섯 단백질 가수분해 15
5) 세포 배양 15
2. 실험방법 16
1) DPPH radical 소거능 16
2) MTT assay 16
3) Type I collagen 합성 17
4) NO assay 17
5) Tyrosinase 억제 17
6) Realtime PCR 18
3. 상황버섯 가수분해물의 간이 임상평가 19
4. 통계 분석 20
III. 결과 21
1. 상황버섯 추출 21
1) 상황버섯 초임계 추출 21
2) 상황버섯 단백질 추출 21
3) 상황버섯 단백질 가수분해 23
2. 상황버섯 가수분해물의 세포평가 29
1) DPPH radical 소거능 29
2) Type I collagen 합성 31
3) NO assay 35
4) 상황버섯 가수분해물의 Tyrosinase 억제에 미치는 영향 39
5) HAS2, 3 mRNA 발현에 미치는 영향 41
3. 상황버섯 가수분해물의 간이 임상평가 44
IV. 고찰 51
V. 결론 58
VI. References 59
Abstract 67
Table 1. Condition of supercritical extraction from... 14
Table 2. Realtime PCR Primer Sequences 19
Table 3. Inter-group comparison test statistics (A versus B) 45
Table 4. Inter-group comparison test statistics (C versus D) 45
Fig. 1. Process flow diagram for supercritical extraction from... 14
Fig. 2. Accumulation of extracted protein yield from Phellinus igniarius 22
Fig. 3. Protein from Phellinus igniarius 22
Fig. 4. Chromatography of hydrolysate by papain over time on ESI-MS 23
Fig. 5. Chromatography of hydrolysate by bromelain over time... 24
Fig. 6. Chromatography of hydrolysate by enzyme mixture over... 25
Fig. 7. Chromatography of comparison 27
Fig. 8. Hydrolysate from Phellinus igniarius by enzyme mixture 28
Fig. 9. Effect of hydrolysate from Phellinus igniarius on DPPH... 30
Fig. 10. Effect of hydrolysate from Phellinus igniarius on cell viability 32
Fig. 11. Expression of type I collagen on CCD-986sk cells by... 34
Fig. 12. Effect of hydrolysate from Phellinus igniarius on cell viability 36
Fig. 13. Inhibition of nitric oxide production on RAW 264.7 cells... 38
Fig. 14. Inhibition of tyrosinase by hydrolysate from Phellinus igniarius 40
Fig. 15. Effect of hydrolysate from Phellinus igniarius on HAS2... 42
Fig. 16. Effect of hydrolysate from Phellinus igniarius on HAS3... 43
Fig. 17. Moisturizing ability on skin of subject 1 by hydrolysate... 46
Fig. 18. Moisturizing ability on skin of subject 2 by hydrolysate... 47
Fig. 19. Moisturizing ability on skin of subject 3 by hydrolysate... 48
Fig. 20. Moisturizing ability on skin of subject 4 by hydrolysate... 49
Fig. 21. Moisturizing ability on skin of subject 5 by hydrolysate... 50