Title Page
Contents
List of Abbreviations 9
Abstract 10
Introduction 11
1. Experimental 16
1.1. Materials and reagents 16
1.2. Synthesis 17
1.3. General fluorescence measurements TPE-AH-OH and abbreviation 20
2. Results and Discussions 22
2.1. Design, Optimization of TPE-Based Dipeptide probe for Hg2+& CH3Hg+[이미지참조] 22
2.2. Characterization of TPE-AH-OH 24
2.3. Fluorescence response of TPE-AH-OH 29
2.4. Screening of TPE-Based Dipeptide Sensors for Hg2+& CH3Hg+[이미지참조] 32
2.5. Fluorescence Study in the Presence of Other Metal Ions and at Different pH 45
2.6. Binding affinity and binding stoichiometry of the probe for Hg2+ and CH3Hg+[이미지참조] 47
2.7. Detection of Mercury Ions in Tap Water and Ground water Using TPE-AH-OH 58
2.8. Effect of pH on the Fluorescence Response of 1 to Hg2+[이미지참조] 60
2.9. Reversible Test for Hg2+ using Glutathione[이미지참조] 63
3. Conclusions 65
4. References 66
Figure 1. Structures of TPE-based peptidyl probes. 15
Figure 2. HPLC spectrum of TPE-AH-OH 25
Figure 3. ESI-MS spectrum of TPE-AH-OH 26
Figure 4. High resolution mass spectrum of TPE-AH-OH 27
Figure 5. ¹H-NMR spectra of TPE-AH-OH 28
Figure 6. Schematic illustration: the reaction of TPE-AH-OH 30
Figure 7. Fluorescence emission spectra of TPE-AH-OH (3 μM)... 31
Figure 8. Fluorescence emission spectra of (a) TPE-AH-OH, (3... 44
Figure 9. Emission response of TPE-AH-OH (3 μM) in the presence of Hg2+...[이미지참조] 46
Figure 10. Job's plot for TPE-AH-OH with Hg2+ in aqueous...[이미지참조] 48
Figure 11. ESI-MS data of TPE-AH-OH (20 μM) in the presence... 50
Figure 12. ESI-MS data of TPE-AH-OH (20 μM) in the presence... 51
Figure 13. Non-linear least square fitting of the emission... 52
Figure 14. Linear intensity change of TPE-AH-OH (3 μM) as a... 55
Figure 15. Partial ¹H NMR spectra (400 MHz) of 1 (5 mM) in the... 57
Figure 16. Fluorescence intensity at 480nm of TPE-AH-OH (3... 61
Figure 17. Fluorescence intensity at 480 nm of TPE-AH-OH (3... 62
Figure 18. Emission spectra of TPE-AH-OH (3 μM) with Hg2+ (4 μM)...[이미지참조] 64
Scheme 1. Proposed binding mode of TPE-AH-OH with Hg2+ &...[이미지참조] 14
Scheme 2. Example of solid phase synthesis of peptidyl probe... 18
Scheme 3. Synthesis of TPE 19