표제지
ABSTRACT
목차
1. Introduction 7
2. Interdigitated Co-culture Experiment 9
2.1. Introduction 9
2.2. Materials and Methods 11
2.2.1. Cell culture 11
2.2.2. Interdigitated disk fabrication 12
2.2.3. Invasion assay 13
2.2.4. Western blot assay 14
2.2.5. Gelatin zymography 15
2.3. Results and Discussion 17
2.3.1. Time-dependent migration of trophoblast 19
2.3.2. Hypoxia-induced hypoxic induced factor-1 alpha expression 22
2.3.3. Hypoxia-induced matrix metalloproteinase expression 24
3. Placenta-on-a-Chip 27
3.1. Introduction 27
3.2. Materials and Methods 29
3.2.1. Cell culture 29
3.2.2. Device design and fabrication 30
3.2.3. Cell co-culture in the device 31
3.2.4. Hypoxia formation by oxygen scavenger 33
3.3. Results and Discussion 34
3.3.1. Regulated oxygen concentration 34
3.3.2. Trophoblast invasion assay 35
4. Conclusions 37
5. References 39