표제지
국문 초록
Abstract
목차
1. 서론 13
2. 재료 및 방법 16
시료 채집 및 환경 정보 측정 16
희석-소멸 배양 실험 20
DNA 추출 및 16S rRNA 유전자 증폭 23
16S rRNA Amplicon Sequencing. 25
3. 실험결과 29
1. 희석 소멸 고효율 배양법을 사용한 지하수 군집구조 분석 및 배양 29
1-1. 희석-소멸 고효율 배양 (High-Throughput Culturing, HTC) 29
1-2. 지하수의 난배양성 세균 배양 43
2. 유전체 분석 46
3. 16S rRNA amplicon sequencing 분석 50
4. 고찰 58
5. 참고문헌 60
Table 1. Information for sampling sites. 17
Table 2. Information for sampling dates. 18
Table 3. Physicochemical parameters of the groundwater samples collected from sites #46 and #26. 19
Table 4. Organic and inorganic components added to the filtered and autoclaved... 21
Table 5. Primers and barcodes used to amplify 16S rRNA gene 26
Table 6. Summary of HTC experiments. 32
Table 7. Phylogenetic analysis of HTC isolates based on 16s rRNA gene sequences. 33
Table 8. General genome characteristics of the novel IMCC strains. 48
Table 9. Relative abundance of prokaryotic groups in the groundwater samples analyzed by amplicon sequencing. 54
Figure 1. Neighbor-joining phylogenetic trees based on 16S rRNA gene sequences... 37
Figure 2. Phylogenetic trees showing the position of phylum Bacteroidota strains... 38
Figure 3. Phylogenetic trees showing the position of phylum Chloroflexi strains... 39
Figure 4. Phlyogenetic trees showing the position of phylum Planctomycetota strains... 40
Figure 5. Phylogenetic trees showing the position of order Sphingobacteriales strains... 41
Figure 6. Phylogenetic trees showing the position of acl clade strains cultured in this... 42
Figure 7. Growth curves of the IMCC strains for which genome sequencing was... 45
Figure 8. Genome-based inference on the metabolic potential of 9 bacterial strains.... 49
Figure 9. Prokaryotic community structure of groundwater samples analyzed by 16S... 53
Figure 10. Principal coordinate analysis (PCoA) plot of 10 groundwater samples... 55
Figure 11. Non-metric multidimensional scaling (NMDS) plot of 10 groundwater... 56
Figure 12. Alpha-diversity indices of 10 groundwater samples collected from... 57