Title Page
Contents
Abstract 8
Korean Abstract (국문 요약) 9
1. Introduction 10
2. Experimental 13
2.1. Materials 13
2.2. Antibody immobilization with SAMs 14
2.3. Impedimetric EIS measurement for OTA detection 14
2.4. OTA detection test in instant coffee samples 15
3. Results and discussion 16
3.1. Immobilization of antibody on gold electrode 16
3.2. Electrochemical performance evaluation of OTA sensor 17
3.3. OTA detection in coffee product 24
4. Conclusion 26
5. Reference 28
Table 1. Difference of Rct value obtained through simulation of equivalent circuit model[이미지참조] 19
Table 2. Difference of Rct value obtained through simulation of equivalent circuit model of 50 mM...[이미지참조] 22
Table 3. Difference of Rct value obtained through simulation of equivalent circuit model of instant...[이미지참조] 25
Figure 1. SEM images of A) bare SPE-Au, B) after 3-MPA, MCH, and EDC-NHS coupling reaction,... 17
Figure 2. A) Nyquist plot and B) CV graph of depending on OTA concentration in 5 mM Fe(CN)63-. 18
Figure 3. A) Rct-R0 vs OTA concentration graph, and B) Rct-R0 vs OTA concentration graph with OTA...[이미지참조] 19
Figure 4. SEM images of OTA sensors after OTA incubation in A) 0.1, B) 1, C) 10, and D) 100 ng/ml. 20
Figure 5. A) Nyquist plot and B) CV graph of depending on OTA high concentration in 50 mM Fe(CN)63-. 20
Figure 6. A) Rct-R0 vs OTA concentration graph, and B) Rct-R0 vs OTA concentration graph Rct-R0 of...[이미지참조] 21
Figure 7. SEM images of OTA sensors after OTA incubation in A) 0, B) 100, C) 200, D) 500, E) 750,... 23
Figure 8. A) Nyquist plot and B) CV graph of instant coffee with and without OTA in 5 mM Fe(CN)63-. 24
Figure 9. SEM images of OTA sensors of incubation in A) instant coffee without OTA and B) with OTA. 25