표제지
목차
Abstract 7
Ⅰ. 서론 8
Ⅱ. 재료 및 방법 10
1. 사용 균주와 균주 Screening 실험 10
2. 균막 형성을 위한 배양 실험 10
3. 배지 최적화 11
4. 무작위 돌연변이 제작 11
5. CRISPR-Cas9을 이용한 유전자 편집 11
Ⅲ. 결과 13
1. 버섯균주의 Screening 13
2. 톱밥배지에서의 배양결과 13
3. 액체배지에서의 배양결과 16
4. 2nd Screening[이미지참조] 18
5. 2nd Screening 종들의 균막 SEM 분석[이미지참조] 18
6. 반고체배지에서의 균막 형성 23
7. 영지버섯 평판고체 배양 27
8. 균막 형성 배지 최적화 29
9. 무작위 돌연변이와 표적 유전자의 유전자편집 35
Ⅳ. 고찰 39
Ⅴ. 참고문헌 41
Table. 1. 1st screening of fungal strains.[이미지참조] 15
Table. 2. 2nd Screening of fungal strains[이미지참조] 21
Table. 3. Fungal mat properties and final pH for each incubation condition in semi-solid medium 24
Fig. 1. 1st screening in sawdust culture[이미지참조] 14
Fig. 2. 1st screening in liquid culture[이미지참조] 17
Fig. 3. 2nd screening in liquid culture[이미지참조] 19
Fig. 4. 2nd screening in sawdust culture[이미지참조] 20
Fig. 5. SEM analysis of the fungal mat 22
Fig. 6A. Fungal strains in each incubation condition 25
Fig. 6B. Fungal strains in each incubation condition 26
Fig. 7. Scale-up with solid medium 28
Fig. 8. Mycelial propagation of different concentration of agar 31
Fig. 9. Mycelial propagation of different carbon and nitrogen sources and concentration of skim milk 32
Fig. 10. Final pH by carbon and nitrogen sources 33
Fig. 11. Mycelial propagation of selected carbon and nitrogen sources 34
Fig. 12. Generation of mutant strains of Ganoderma lucium and mycelial growth 36
Fig. 13. Characterization of hydrophobin 38