표제지
목차
Abstract 11
I. 서론 12
II. 재료 및 방법 13
1. 해수 순치 조건에 따른 조직과 대사 변화 13
1.1. 실험조건 13
1.2. 조직학적 변화 16
1.3. Na+, K+ ATPase 검사 21
2. 해수 순치 후 수온증가에 따른 연어의 폐사 원인 규명 23
2.1. 사육조건 23
2.2. 질병 검사 26
2.3. 혈구 조성 검사 28
2.4. 병리 조직학적 검사 32
III. 결론 33
1. 해수 순치 조건에 따른 조직과 대사 변화 33
1.1. 염분농도 변화에 따른 폐사율 33
1.2. 조직학적 변화 35
1.3. Na+, K+ ATPase 검사 41
2. 해수 순치 후 수온증가에 따른 연어의 폐사 원인 규명 44
2.1. 사육 수온에 따른 폐사율 44
2.2. 질병 검사 46
2.3. 혈구 조성 검사 60
2.4. 병리 조직학적 검사 67
IV. 고찰 76
1. 해수 순치 조건에 따른 조직과 대사 변화 76
2. 해수 순치 후 수온증가에 따른 연어의 폐사 원인 규명 79
V. 요약 82
1. 연구의 결과 82
2. 연구의 시사점 82
3. 연구의 한계와 추후 방향 82
VI. 참고문헌 83
Table 1. Experimental conditions (water temperature, daily salinity change concentration) 15
Table 2. Na+, K+ ATPase activity measurement method 22
Table 3. Test items and number of samples according to the experiment date 25
Table 4. Disease Test Results 48
Figure 1. 90L circulation filtration tank 14
Figure 2. Schematic diagram of the production process of tissue specimens... 17
Figure 3. Quantitative analysis of the epithelial thickness of salmon gill lamella 19
Figure 4. Quantitative analysis of salmon gill salt cells and mucus cells 20
Figure 5. OTC and bag filter used in the experiment 24
Figure 6. Types and characteristics of blood cells in fish blood 29
Figure 7. Blood smear and staining of subjects 30
Figure 8. Granulocyte and agranular and bacterial infections in the blood 31
Figure 9. Mortality rate of young salmon according to conditions 34
Figure 10. Changes in the thickness of the epithelial layer of salmon gills... 36
Figure 11. Changes in distribution of gill mucus cells in salmon according to... 38
Figure 12. Changes in the distribution rate of gill chloride cells in salmon... 40
Figure 13. Na+, K+ ATPase activity in salmon according to salt concentration... 42
Figure 14. Na+, K+ ATPase activity in salmon according to salt concentration... 43
Figure 15. Salmon mortality according to water temperature 45
Figure 16. Base sequence analysis result of bacteria (pale cream colony)... 49
Figure 17. Results of sequencing of bacteria (pale cream colony) isolated from... 50
Figure 18. Base sequence analysis result of bacteria (pale cream colony)... 51
Figure 19. Base sequence analysis result of bacteria (pale cream colony)... 52
Figure 20. Base sequence analysis result of bacteria (pale cream colony)... 53
Figure 21. Base sequence analysis result of bacteria (yellow colony) isolated... 54
Figure 22. Base sequence analysis result of bacteria (pale cream colony)... 55
Figure 23. Homology analysis between isolated bacteria 57
Figure 24. Virus test result of kidney spleen tissue 59
Figure 25. Wright-Giemsa stained granular leukocytes 61
Figure 26. Blood composition of young salmon exposed to high water temperature 62
Figure 27. Whole blood cell composition (left) and white blood cell... 63
Figure 28. The percentage of white blood cells in young salmon exposed to... 65
Figure 29. Comparison of white blood cell composition in the blood of young... 66
Figure 30. Normal salmon liver tissue 68
Figure 31. Histological changes in salmon liver under high water temperature conditions 69
Figure 32. Histological structure of control salmon gills 71
Figure 33. Histological changes of salmon gills under high temperature conditions 72
Figure 34. Histological structure of the salmon control kidney 74
Figure 35. Histological changes of salmon kidney in high water temperature conditions 75