Title Page
Contents
Abstract 8
1. Introduction 10
2. Materials and Methods 18
2.1. Microorganisms Culture and Selection 18
2.2. Biodegradation of Nile perch wastewater 19
2.3. Analyses 21
2.4. Phytotoxicity test 22
2.5. Hydroponic culture 23
2.6. Antioxidant activity 24
2.6.1. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay 24
2.6.2. 2, 2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization assay 25
2.6.3. Hydroxyl radical scavenging activity 26
2.7. Statistical analysis 27
3. Results and Discussion 28
3.1. Microorganisms Culture and Selection 28
3.2. Biodegradation of Nile perch wastewater 30
3.3. Phytotoxicity test 33
3.4. Hydroponic culture 35
3.5. Antioxidants activity of biodegraded Nile perch wastewater 40
3.5.1. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay 41
3.5.2. 2, 2'-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) ABTS radical cation decolorization assay 43
3.5.3. Hydroxyl radical scavenging activity 46
4. Conclusion 49
6. References 53
Table. 1. Proximate composition of young and adult Nile perch skins 20
Table. 2. The quantity of amino acids presents in the scale and skin collagen of young and... 20
Table. 3. The hydroponic culture findings of barley on 72 h culture medium of Nile perch wastewater 35
Table. 4. Comparison of fertilizing ability of biofertilizers from different fishery... 39
Table. 5. Antioxidant activities comparison of fishery hydrolysate and wastewater with... 48
Fig. 1. Clear zone diameter of microorganism for protease activity level. The data... 28
Fig. 2. CODcr and TN (mg/l), CFU (ml/l), proteolytic activity (cm) and pH changes of... 32
Fig. 3. Germination Index of biodegraded Nile perch wastewater with 2, 10, 50, 100, 500... 35
Fig. 4. DPPH free radical scavenging activity of biodegraded Nile perch wastewater... 42
Fig. 5. ABTS radical scavenging activity of biodegraded Nile perch wastewater... 45
Fig. 6. Hydroxyl radical scavenging activity of biodegraded Nile perch wastewater... 47