표제지
목차
요약 10
I. 서론 12
1.1. 미세플라스틱의 이론적 정의 12
1.2. 미세플라스틱의 발생형태와 영향 13
1.3. 연구목적 16
II. 실험 재료 및 방법 17
2.1. 대상 미세플라스틱 17
2.2. UV 조사 20
2.3. 미세플라스틱의 특성화 21
2.3.1. ATR-FTIR 표면변화 21
2.3.2. SEM 표면변화 21
2.4. 토양시료채취 및 특성 분석 21
2.5. 미생물 효소활성 측정 23
2.5.1. 토양 효소활성 측정원리 23
2.5.2. Fluorescein diaetate activity 24
2.6. 미생물 군집구조 분석 25
2.6.1. DNA 추출 및 PCR, pyrosequencing 25
III. 실험결과 및 고찰 27
3.1. ATR-FTIR 표면변화 27
3.2. SEM 표면변화 35
3.3. 미생물 활성도 37
3.4. 미생물 군집 해석 38
3.4.1. 미생물 집단의 분류학적 분포 38
3.4.2. 시퀀싱 결과 및 알파, 베타 다양성 지수 지정 40
3.4.3. 미생물 다양성 지표 결과 41
IV. 결론 45
참고문헌 47
Abstract 52
Table 1.1. Microplastic effect in soil studied in China 15
Table 2.1. Properties of plastics for each composition 19
Table 2.2. Physicochemical properties of soil used in this study 22
Table 2.3. Experimental information on soil microbial enzyme activity 24
Table 3.1. Types of vibrations for wavelengths identified in this study 34
Fig 1.1. Definition of microplastics according to the size proposed by... 12
Fig 1.2. Type of plastic released into the environment(Boucher and... 14
Fig 2.1. Experimental progress 17
Fig 2.2. Polymer extruder(LME-TUR) 18
Fig 2.3. UV Aging chamber 20
Fig 2.4. No. 10(〈 2 mm) sieving and drying for 7 d 22
Fig 2.5. Fluorescein production process as a fluorescent substance... 23
Fig 3.1. Aged MPs absorbance and after subtracting the Virgin... 28
Fig 3.2. Peaks increased and identified in each wavelength... 29
Fig 3.3. Peaks increased and identified in each wavelength... 30
Fig 3.4. Peaks increased and identified in each wavelength... 31
Fig 3.5. Peaks increased and identified in each wavelength... 32
Fig 3.6. Peaks increased and identified in each wavelength... 33
Fig 3.7. SEM images of virgin(upper) and aged(lower) microplastics 36
Fig 3.8. Visually changed surface after aged microplastics 36
Fig 3.9. Fluorescein diacetate activity in soils with or without plastic... 37
Fig 4.0. Changes in bacterial communities at the (a) Class and (b)... 39
Fig 4.1. Rare OTU(Singleton and Doubleton) Diversity Index... 42
Fig 4.2. Shannon, NPShanon(non-parametric Shannon) is an index... 43
Fig 4.3. Principal component analysis of bacterial communities 44