표제지
목차
ABBREVIATIONS 12
국문초록 13
I. 서론 16
II. 재료 및 방법 19
1. 실험 재료 19
(1) 식물 재료 19
(2) 세포주 19
(3) 시약 및 배지 19
2. 실험방법 20
(1) 포포나무 잎 추출, 분획 및 분리 20
(2) 포포나무 잎 추출물 및 분획물의 항산화 활성 측정 29
(3) 포포나무 잎 추출물 및 분획물의 활성성분 분석 33
(4) 통계분석 35
III. 결과 및 고찰 36
1. 포포나무 잎 추출물 및 분획물의 수율 및 성분 함량 36
(1) 추출물과 분획물의 수율 36
(2) 총 페놀성 화합물 및 flavonoid 함량 38
(3) Acetogenin류 분석 40
2. 포포나무 잎 추출물과 분획물의 항산화 활성 43
(1) DPPH radical 소거능 43
(2) ABTS radical 소거능 50
(3) Nitric oxide 생성 억제 활성 56
3. 포포나무 잎 추출물 성분 분석 62
(1) EF-2의 주성분 동정 62
(2) EF-3의 주성분 동정 65
(3) EF-5의 주성분 동정 68
참고문헌 71
Abstract 83
Table 1. HPLC separation condition 22
Table 2. Mass spectroscopy parameters for analysis of acetogenins in... 27
Table 3. Multiple reaction monitoring parameters for analysis of acetogenins 28
Table 4. LC-UVD-MS analysis condition 34
Table 5. Yields of Asimina triloba leaf extract and its fractions 37
Table 6. Total phenolic compound and flavonoid contents in Asimina triloba... 39
Table 7. DPPH radical scavenging ability of Asimina triloba leaf extract... 46
Table 8. DPPH radical scavenging ability of ethylacetate fraction and its... 49
Table 9. ABTS radical scavenging ability of Asimina triloba leaf extract... 53
Table 10. ABTS radical scavenging ability of ethylacetate fraction and its... 55
Figure 1. Preparation of fractions depend on solvent polarity and production... 21
Figure 2. LC profile of ethylacetate fraction obtained from Asimina triloba... 23
Figure 3. Standard curves of tannic acid [A] and rutin [B] by colorimetric... 25
Figure 4. MRM chromatograms of acetogenins in Asimina triloba leaf... 41
Figure 5. MRM chromatograms of acetogenins in ethylacetate sub-fractions... 42
Figure 6. DPPH radical scavenging ability of Asimina triloba leaf extract... 45
Figure 7. DPPH radical scavenging ability of ethylacetate fraction and its... 48
Figure 8. ABTS radical scavenging ability of Asimina triloba leaf extract... 52
Figure 9. ABTS radical scavenging ability of ethylacetate fraction and its... 54
Figure 10. Effect of Asimina triloba leaf extract and its fractions in LPS... 57
Figure 11. Effect of ethylacetate fraction and its sub-fractions isolated from... 58
Figure 12. Nitric oxide production inhibit effect of Asimina triloba leaf... 60
Figure 13. Nitric oxide production inhibit effect of ethylacetate fraction and... 61
Figure 14. LC chromatogram (A) and LC-MS/MS spectra (B) of EF-2... 63
Figure 15. LC chromatogram of EF-2 isolated from ethylacetate fraction of... 64
Figure 16. LC chromatogram (A) and LC-MS/MS spectra (B) of EF-3... 66
Figure 17. LC chromatogram of EF-3 isolated from ethylacetate fraction of... 67
Figure 18. LC chromatogram (A) and LC-MS/MS spectra (B) of EF-5... 69
Figure 19. LC chromatogram of EF-5 isolated from ethylacetate fraction of... 70