표제지
목차
I. 서론 7
II. 재료 및 방법 10
1. 차나무 재배지로부터 당화 박테리아 분리 10
2. 당화 미생물의 동정 10
3. 당화 미생물의 효소 활성측정 13
4. Zymogram assay 14
III. 결과 및 고찰 15
1. 차나무 재배지에서 목질바이오매스 분해 박테리아 분리 15
2. 16S rRNA 유전자를 이용한 목질바이오매스 분해 박테리아 동정 17
3. CMCase의 Zymogram 분석 23
4. 목질바이오매스 분해 박테리아의 생장 특성 25
5. CMCase와 Xylanase의 최적 온도 및 pH 30
6. CMCase와 Xylanase의 온도 안정성 36
IV. 결론 41
V. 참고문헌 43
국문초록 47
ABSTRACT 49
Figure 1. Screening of cellulolytic bacteria from Camellia sinensis cultivated soil. 16
Figure 2. Nucleotide sequence of 16S rRNA gene from Bacillus sp. CS1 isolated from... 19
Figure 3. Nucleotide sequence of 16S rRNA gene from Bacillus sp. CS2 isolated from... 20
Figure 4. Nucleotide sequence of 16S rRNA gene from Bacillus sp. CS3 isolated from... 21
Figure 5. A neighbor-joining phylogenetic tree based on the 16S rRNA gene of... 22
Figure 6. Detection of CMCase activity from Bacillus sp. CS1, CS2, and CS3 by... 24
Figure 7. Cell growth and enzymatic activity of CMCase and xylanase produced by... 27
Figure 8. Cell growth and enzymatic activity of CMCase and xylanase produced by... 28
Figure 9. Cell growth and enzymatic activity of CMCase and xylanase produced by... 29
Figure 10. Effect of pH and temperature on the relative activity of CMCase (A) and... 33
Figure 11. Effect of pH and temperature on the relative activity of CMCase (A) and... 34
Figure 12. Effect of pH and temperature on the relative activity of CMCase (A) and... 35
Figure 13. Thermal stability of CMCase (A) and xylanase (B) from Bacillus sp. CS1. 38
Figure 14. Thermal stability of CMCase (A) and xylanase (B) from Bacillus sp. CS2. 39
Figure 15. Thermal stability of CMCase (A) and xylanase (B) from Bacillus sp. CS3... 40