표제지
목차
국문초록 12
I. 서론 14
II. 재료 및 방법 18
1. 재료 18
1) 실험동물 18
2) 작약감초탕 18
3) 5-aminosalicylic acid (5-ASA) 20
4) 침 (Acupuncture needle) 20
5) ELISA Kit 20
6) H&E stain 21
2. 실험 방법 21
1) 군 분리 21
2) Dextrane sulfate 유도 염증성 장질환 유발 22
3) 약물 투여 및 침치료 시행 22
4) 체중 및 DAI(Disease activity index) score 측정 23
5) Histology score (H&E Stain) 23
6) Inflammatory Cytokine 측정 24
7) 대사산물 분석 27
3. 통계 분석 28
III. 실험 결과 29
1. Macrophage activating factor (IL-6, IL-23, TNF-α) 변화 29
2. Th1 cytokine (IFN-γ) 변화 30
3. Th2 cytokine (IL-5, IL-13) 변화 31
4. 대장 길이 및 H&E histology score 변화 32
5. 체중 및 DAI(Disease activity index) score 변화 34
1) 체중 변화 34
2) DAI(Disease activity index) score 변화 35
6. 대사산물 변화 36
IV. 고찰 43
V. 결론 54
참고문헌 56
Abstract 63
Table 1. HPLC Gradient Flow Condition for the Analysis of Jakyakgamcho-tang... 19
Table 2. Cytokine ELISA Kit information 21
Table 3. Hematoxylin & Eosin stain information 21
Table 4. DAI(Disease activity index) score calculation of IBD 23
Table 5. Histology score calculation of IBD 24
Table 6. Metabolic effects of Dextrane sulfate induced IBD and JGT, Acu and... 52
Fig. 1. Cytokines in the pathogenesis of IBD. 15
Fig. 2. Peak of the main components by HPLC Analysis of Jakyakgamcho-tang. 19
Fig. 3. Determination of Glycyrrhizic Acid and Paeoniflorin Contents in... 20
Fig. 4. Acupoint CV12, ST25 and CV4... 22
Fig. 5. Experimental IBD model protocol for Jakyakgamcho-tang and Acupuncture... 23
Fig. 6. Effect of combination treatments on Macrophage activating factor in... 29
Fig. 7. Effect of combination treatments on Cronhn's pathogenesis in... 30
Fig. 8. Effect of combination treatments on ulcerative colitis pathogenesis in 3%... 31
Fig. 9. (L) Representative images by H&E staining displaying colon segments on the... 32
Fig. 10. (Upper) Representative images by H&E staining displaying colon segments... 33
Fig. 11. (Upper)Full period weight variation, (Under)Last day weight change. 34
Fig. 12. (Upper)Full period DAI score variation, (Under)Last day DAI score change. 35
Fig. 13. PLS-DA score plot. 36
Fig. 14. PLS-DA score plots for all groups(Upper; normal, control, 5-ASA, JGT, Acu... 37
Fig. 15. (L) PLS-DA scores plots for Normal and control groups derived from GC-MS... 38
Fig. 16. Metabolic product contributing to the difference between Normal and Control. 38
Fig. 17. PLS-DA scores plots for Control and 5-ASA groups derived from GC-MS data... 39
Fig. 18. PCA score plots for Control, JGT, Acu and JGT+Acu treated groups derived... 39
Fig. 19. (L) PLS-DA scores plots for Control and JGT derived from GC-MS data of... 40
Fig. 20. Metabolic product contributing to the difference between Control and JGT. 40
Fig. 21. (L) PLS-DA scores plots for control and Acu derived from GC-MS data of... 41
Fig. 22. Metabolic product contributing to the difference between Control and Acu. 41
Fig. 23. (L) PLS-DA scores plots for control and JGT+Acu derived from GC-MS data... 42
Fig. 24. Metabolic product contributing to the difference between control and JGT+Acu. 42
Fig. 25. Redundancy and specificity in IL-1, IL-6 and... 47
Fig. 26. IL-23 Cytokines in the pathogenesis of IBD. 48