표제지
목차
국문초록 9
I. 서론 11
II. 재료 및 방법 13
1. 재료 13
1) 약재 13
2) 세포주 13
2. 방법 13
1) 시료 제조 13
2) 세포 생존율 측정 14
3) Melanin 생합성율 측정 14
4) 시험관 내 tyrosinase 활성 억제율 측정 15
5) 세포 내 tyrosinase 활성 억제율 측정 15
6) Superoxide dismutase (SOD) 유사활성도 측정 16
7) Polymerase chain reaction (PCR)를 활용한 실험방법 17
3. 통계처리 18
III. 성적 19
1. 세포 생존율에 미치는 효과 19
2. Melanin 생성 억제 효과 21
3. α-MSH에 의해 촉진된 melanin 생합성율 억제 효과 23
4. 시험관 내에서의 농도별 tyrosinase 억제 효과 25
5. 세포 내에서의 tyrosinase 억제 효력 27
6. α-MSH로 촉진된 세포 내에서의 tyrosinase 저해 효과 29
7. Tyrosinase 발현에 미치는 영향 31
(1) α-MSH를 처리하지 않은 경우 31
(2) α-MSH를 처리한 경우 33
8. TRP-2 발현에 미치는 영향 35
(1) α-MSH를 처리하지 않은 경우 35
(2) α-MSH를 처리한 경우 37
9. MITF 발현에 미치는 영향 39
(1) α-MSH를 처리하지 않은 경우 39
(2) α-MSH를 처리한 경우 41
10. Superoxide dismutase (SOD) 유사활성에 미치는 효과 43
IV. 고찰 45
V. 결론 53
참고문헌 54
ABSTRACT 61
Table 1. List of primer sequences used for RT-PCR analysis. 18
Table 2. The effects of RRE on proliferation rates in B16F10 cells. 19
Table 3. The inhibitory effects of RRE on melanin synthesis. 21
Table 4. The inhibitory effects of RRE on melanin synthesis in... 23
Table 5. The inhibitory effects of RRE on tyrosinase activity... 25
Table 6. The inhibitory effects of RRE on tyrosinase activity... 27
Table 7. The inhibitory effects of RRE on tyrosinase synthesis in... 29
Table 8. The effect of RRE on tyrosinase level. 31
Table 9. The effect of RRE on tyrosinase level by induced... 33
Table 10. The effect of RRE on TRP-2 level. 35
Table 11. The effect of RRE on TRP-2 level by induced α-MSH. 37
Table 12. The effect of RRE on MITF level. 39
Table 13. The effect of RRE on MITF level by induced α-MSH. 41
Table 14. The effect of RRE on SOD-like activity. 43
Figure 1. Effects of RRE on proliferation rates in B16F10 cells. 20
Figure 2. Inhibitory effects of RRE on melanin synthesis. 22
Figure 3. Inhibitory effects of RRE on melanin synthesis in α-MSH... 24
Figure 4. Inhibitory effects of RRE on tyrosinase activity... 26
Figure 5. Inhibitory effects of RRE on tyrosinase activity... 28
Figure 6. Inhibitory effects of RRE on tyrosinase synthesis in... 30
Figure 7. Effects of RRE on mRNA expression of tyrosinase in... 32
Figure 8. Effects of RRE on mRNA expression of tyrosinase in... 34
Figure 9. Effects of RRE on mRNA expression of TRP-2... 36
Figure 10. Effects of RRE on mRNA expression of TRP-2 in... 38
Figure 11. Effects of RRE on mRNA expression of MITF... 40
Figure 12. Effects of RRE on mRNA expression of MITF in α-MSH... 42
Figure 13. Effect of RRE on SOD-like activity. 44
Figure 14. Mechanisms of the formation of melanin pigments by... 47