표제지
목차
국문초록 16
I. 서론 18
1-1. 연구의 배경 18
1-2. 연구의 목적 22
1-3. 연구내용 및 방법 23
1-3-1. 연구내용 23
1-3-2. 연구방법 23
II. 문헌조사 25
2-1. 분변토 25
2-1-1. Vermicomposting의 배경 25
2-1-2. Vermicast의 기능 27
2-2. 가축분뇨발효액 28
2-2-1. 가축분뇨발효액의 상용화 배경 28
2-2-2. 가축분뇨발효액의 기능 31
2-3. 근권 토양미생물 39
2-3-1. 신선채소의 오염미생물 39
2-3-2. 퇴비의 미생물 41
III. 실험 43
3-1. 분변토 처리 유식물체 생육 시험 43
3-1-1. 분변토 처리 작물생육 시험 설계 43
3-1-2. 유식물체 생육양상 조사 43
3-2. 가축분뇨발효액 처리 유식물체 생육시험 48
3-2-1. 가축분뇨발효액 처리 작물생육 시험 설계 48
3-2-2. 유식물체 생육양상 조사 48
3-3. 분변토 처리 토양 시험 54
3-3-1. 분변토 시료 54
3-3-2. 분변토 처리 포장시험방법 54
3-4. 가축분뇨발효액 처리 토양 시험 56
3-4-1. 가축분뇨발효액 시료 56
3-4-2. 가축분뇨발효액 처리 포장시험방법 56
3-5. 분변토·가축분뇨발효액 처리 토양 미생물 시험 58
3-5-1. 미생물 시험을 위한 처리토양 시료 채취방법 58
3-5-2. 일반미생물 밀도조사 58
3-5-3. 작물생육촉진미생물(PGPB) 밀도조사 59
3-5-4. 특정물질 분해 미생물 밀도조사 60
3-5-5. 병원성미생물 밀도조사 61
IV. 결과 및 고찰 62
4-1. 분변토 처리 작물생육시험 62
4-1-1. 무(청우무) 62
4-1-2. 상추(청치마) 63
4-1-3. 토마토(썬글로브) 64
4-1-4. 오이(백봉다다기) 65
4-1-5. 배추(노랑쌈배추) 66
4-2. 가축분뇨발효액 처리 작물생육시험 67
4-2-1. 무(청우무) 67
4-2-2. 상추(청치마) 68
4-2-3. 토마토(썬글로브) 69
4-2-4. 오이(백봉다다기) 70
4-2-5. 배추(노랑쌈배추) 71
4-3. 분변토 처리 미생물 조사 72
4-3-1. 분변토 내 미생물상 72
4-3-2. 분변토 처리 토양 내 일반미생물의 밀도변화 75
4-3-3. 분변토 처리 토양 내 작물생육미생물(PGPB)의 밀도변화 84
4-3-4. 분변토 처리 토양 내 특정물질 분해미생물의 밀도변화 93
4-3-5. 분변토 처리 토양 내 병원성 미생물의 밀도변화 105
4-4. 가축분뇨발효액 처리 미생물 조사 결과 108
4-4-1. 가축분뇨발효액 내 미생물상 108
4-4-2. 가축분뇨발효액 처리 토양 내 일반미생물의 밀도변화 111
4-4-3. 가축분뇨발효액 처리 토양 내 작물생육 촉진 미생물(PGPB)의 밀도 120
4-4-4. 가축분뇨발효액 처리 토양 내 특정물질 분해미생물의 밀도 129
4-4-5. 가축분뇨발효액 처리 토양 내 병원미생물의 밀도 141
V. 결론 144
참고 문헌 148
Abstracts 156
Table 1-1. The present state of livestock breeding in Korea 20
Table 3-1. Sample processing in vermicast 44
Table 3-2. Sample processing in liquid fertilizer 49
Table 4-1. Growth of radish plants after vermicast treatment 62
Table 4-2. Growth of lettuce plants after vermicast treatment 63
Table 4-3. Growth of tomato plants after vermicast treatment 64
Table 4-4. Growth of cucumber plants after vermicast treatment 65
Table 4-5. Growth of cabbage plants after vermicast treatment 66
Table 4-6. Growth of radish plants after liquid fertilizer treatment 67
Table 4-7. Growth of lettuce plants after liquid fertilizer treatment 68
Table 4-8. Growth of tomato plants after liquid fertilizer treatment 69
Table 4-9. Growth of cucumber plants after liquid fertilizer... 70
Table 4-10. Growth of cabbage plants after liquid fertilizer... 71
Fig 3-1. Steps of experiment preparation, seeding preparation, and... 45
Fig 3-2. Preparation of test port after vermicast processing. 46
Fig 3-3. Transplant seeding to experimental ports that processed... 47
Fig 3-4. Steps of experiment preparation, seeding preparation,... 50
Fig 3-5. Growth test in liquid fertilizer. 51
Fig 3-6. Planting plants in test with liquid fertilizer. 52
Fig 3-7. Injection of liquid fertilizer by treatment zone. 53
Fig 3-8. Vermicast treatment test site and test group. 55
Fig 3-9. Vermicast test vinyl house. 55
Fig 3-10. Liquid fertilizer treatment test site and test group. 57
Fig 3-11. Liquid fertilizer test vinyl house. 57
Fig 3-12. Collects top soil. 58
Fig 4-1. Condition(A) and density(B) in Bacteria(a),... 72
Fig 4-2. Condition(A) and density(B) in Bacillus(a),... 73
Fig 4-3. Condition(A) and density(B) in protease(a), amylase(b)... 74
Fig 4-4. Density trend of Bacteria for 90 days treatment of... 75
Fig 4-5. Density trend of Bacteria for 90 days treatment of... 76
Fig 4-6. Change in Bacteria density for 90 days of vermicast... 77
Fig 4-7. Density trend of Actinobacteria for 90 days treatment of... 78
Fig 4-8. Density trend of Actinobacteria for 90 days treatment of... 79
Fig 4-9. Change in Actinobacteria density for 90 days of... 80
Fig 4-10. Density trend of Fungi for 90 days treatment of... 81
Fig 4-11. Density trend of Fungi for 90 days treatment of... 82
Fig 4-12. Change in Fungi density for 90 days of vermicast... 83
Fig 4-13. Density trend of Bacillus for 90 days treatment of... 84
Fig 4-14. Density trend of Bacillus for 90 days treatment of... 85
Fig 4-15. Change in Bacillus for 90 days of vermicast... 86
Fig 4-16. Density trend of Lactobacillus for 90 days treatment of... 87
Fig 4-17. Density trend of Lactobacillus for 90 days treatment of... 88
Fig 4-18. Change in Lactobacillus density for 90 days of... 89
Fig 4-19. Density trend of Pseudomonas fluorescens for 90 days... 90
Fig 4-20. Density trend of Pseudomonas fluorescens for 90 days... 91
Fig 4-21. Change in Pseudomonas fluorescens density for 90 days... 92
Fig 4-22. Density trend of protease-producing bacteria for 90 days... 93
Fig 4-23. Density trend of protease-producing bacteria for 90 days... 94
Fig 4-24. Change in protease-producing bacteria density for 90... 95
Fig 4-25. Density trend of amylase-producing bacteria for 90 days... 96
Fig 4-26. Density trend of amylase-producing bacteria for 90 days... 97
Fig 4-27. Change in amylase-producing bacteria density for 90... 98
Fig 4-28. Density trend of cellulase-producing bacteria for 90... 99
Fig 4-29. Density trend of cellulase-producing bacteria for 90 days... 100
Fig 4-30. Change in cellulase-producing bacteria density for 90... 101
Fig 4-31. Density trend of phosphatase-producing bacteria for 90... 102
Fig 4-32. Density trend of phosphatase-producing bacteria for 90... 103
Fig 4-33. Change in phosphatase-producing bacteria density for 90... 104
Fig 4-34. Density trend Escherichia coli for 90 days treatment... 105
Fig 4-35. Density trend Escherichia coli for 90 days treatment... 106
Fig 4-36. Change in Escherichia coli density for 90 days of... 107
Fig 4-37. Condition(A) and density(B) in Bacteria(a),... 108
Fig 4-38. Condition(A) and density(B) in Bacillus(a),... 109
Fig 4-39. Condition(A) and density(B) in protease(a), amylase(b),... 110
Fig 4-40. Density trend of Bacteria for 90 days treatment of liquid... 111
Fig 4-41. Density trend of Bacteria for 90 days treatment of liquid... 112
Fig 4-42. Change in Bacteria density for 90 days of liquid... 113
Fig 4-43. Density trend of Actinobacteria for 90 days treatment of... 114
Fig 4-44. Density trend of Actinobacteria for 90 days treatment of... 115
Fig 4-45. Change in Actinobacteria density for 90 days of liquid... 116
Fig 4-46. Density trend of Fungi for 90 days treatment of liquid... 117
Fig 4-47. Density trend of Fungi for 90 days treatment of liquid... 118
Fig 4-48. Change in Fungi density for 90 days of liquid fertilizer... 119
Fig 4-49. Density trend of Bacillus for 90 days treatment of liquid... 120
Fig 4-50. Density trend of Bacillus for 90 days treatment of liquid... 121
Fig 4-51. Change in Bacillus density for 90 days of liquid... 122
Fig 4-52. Density trend of Lactobacillus for 90 days treatment of... 123
Fig 4-53. Density trend of Lactobacillus for 90 days treatment of... 124
Fig 4-54. Change in Lactobacillus density 90 days of liquid... 125
Fig 4-55. Density trend of Pseudomonas fluorescens for 90 days... 126
Fig 4-56. Density trend of Pseudomonas fluorescens for 90 days... 127
Fig 4-57. Change in Pseudomonas fluorescens density for 90 days... 128
Fig 4-58. Density trend of protease-producing bacteria for 90 days... 129
Fig 4-59. Density trend of protease-producing bacteria for 90 days... 130
Fig 4-60. Change in protease-producing bacteria density for 90... 131
Fig 4-61. Density trend of amylase-producing bacteria for 90 days... 132
Fig 4-62. Density trend of amylase-producing bacteria for 90 days... 133
Fig 4-63. Change in amylase-producing bacteria density for 90... 134
Fig 4-64. Density trend of cellulase-producing bacteria for 90... 135
Fig 4-65. Density trend of cellulase-producing bacteria for 90... 136
Fig 4-66. Change in cellulase-producing bacteria density for 90... 137
Fig 4-67. Density trend of phosphatase-producing bacteria for 90... 138
Fig 4-68. Density trend of phosphatase-producing bacteria for 90... 139
Fig 4-69. Change in phosphatase-producing bacteria density for 90... 140
Fig 4-70. Density trend of Escherichia coli for 90 days treatment... 141
Fig 4-71. Density trend of Escherichia coli for 90 days treatment... 142
Fig 4-72. Change in Escherichia coli density for 90 days of liquid... 143