표제지
목차
국문 초록 10
I. 서론 12
II. 재료 및 방법 14
1. 재료 14
1) 시료 14
2) 실험동물 및 사육 14
3) 시약 14
4) 기기 15
2. 방법 15
1) 시료 추출 15
2) HPLC 분석 15
3) 중금속 검사 16
4) 아토피 피부염 유발 및 시료처리 16
5) 혈청 분리 및 등 조직 채취 17
6) 육안적 평가 17
7) 혈액 내 바이오마커 측정 17
8) 면역세포 분석 18
9) 조직 내 바이오마커 측정 19
10) 조직학적 검사 19
3. 통계처리 20
III. 실험결과 21
1. HPLC 분석 21
2. 중금속 함량 22
3. 육안적 평가 23
4. 혈액 내 바이오마커 측정 24
1) Immunoglobulin E (IgE) 24
2) Histamine 25
3) Cytokine 26
5. 면역세포 분석 34
1) White blood cell 34
2) Monocyte 35
3) Neutrophil 36
4) Eosinophil 37
5) Basophil 38
6) Lymphocyte 39
6. 조직 바이오마커 측정 40
1) Filaggrin 40
2) VEGF 41
3) TGF-β1 42
4) EGF 43
7. 조직학적 검사 44
1) 피부 두께 44
2) 호산구 침윤 45
IV. 고찰 46
V. 결론 50
참고문헌 52
ABSTRACT 56
Table 1. The Prescription of GMD 14
Table 2. The Sequences of Primers for Real-Time PCR 20
Table 3. Content of Pb, As, Cd and Hg in GMD 22
Fig. 1. HPLC analysis of GMD. 21
Fig. 2. Dermatitis score in the DNCB-caused AD mice. 23
Fig. 3. The effects of GMD on IgE level in the DNCB-caused AD... 24
Fig. 4. The effects of GMD on histamine level in the DNCB-caused... 25
Fig. 5. The effects of GMD on IL-1β level in the DNCB-caused AD... 26
Fig. 6. The effects of GMD on IL-2 level in the DNCB-caused AD... 27
Fig. 7. The effects of GMD on IL-4 level in the DNCB-caused AD... 28
Fig. 8. The effects of GMD on IL-5 level in the DNCB-caused AD... 29
Fig. 9. The effects of GMD on IL-6 level in the DNCB-caused AD... 30
Fig. 10. The effects of GMD on IL-10 level in the DNCB-caused AD... 31
Fig. 11. The effects of GMD on TNF-α level in the DNCB-caused... 32
Fig. 12. The effects of GMD on IFN-γ level in the DNCB-caused... 33
Fig. 13. The effects of GMD on white blood cell level in the... 34
Fig. 14. The effects of GMD on monocyte level in the DNCB-caused... 35
Fig. 15. The effects of GMD on neutrophil level in the... 36
Fig. 16. The effects of GMD on eosinophil level in the DNCB-caused... 37
Fig. 17. The effects of GMD on basophil level in the DNCB-caused... 38
Fig. 18. The effects of GMD on lymphocyte level in the... 39
Fig. 19. The effects of GMD on filaggrin mRNA level in the... 40
Fig. 20. The effects of GMD and DCL on VEGF mRNA level in the... 41
Fig. 21. The effects of GMD on TGF-β1 mRNA level in the... 42
Fig. 22. The effects of GMD on EGF mRNA level in the... 43
Fig. 23. The effects of GMD on skin thickness in the DNCB-caused... 44
Fig. 24. The effects of GMD on eosinophil infiltration in the... 45