표제지
목차
ABBREVIATIONS 11
국문초록 12
I. 서론 15
II. 재료 및 방법 19
1. 실험 재료 19
2. 시약 19
3. 세포주 20
4. 실험 방법 20
(1) 비파엽 추출물의 제조 20
(2) 비파엽 추출물 및 주성분의 항산화 활성 검색 21
(3) 비파엽 추출물의 탈과립억제 효과 검색 25
(4) 비파엽 추출물의 성분분석 29
(5) 통계처리 33
III. 결과 및 고찰 34
1. 비파엽 추출물의 수율 34
2. 비파엽 추출물의 항산화 활성 36
(1) DPPH radical 소거능 36
(2) Nitric oxide 생성억제 활성 41
3. RBL-2H3 세포에서 비파엽 추출물의 탈과립억제 효과 47
(1) RBL-2H3 세포의 세포생존율 47
(2) β-Hexosaminidase 방출억제 효과 50
4. 비파엽 추출물의 성분 분석 54
(1) 주성분 확인을 위한 LC profile 분석과 LC-MS 분석 54
(2) LC-MS/MS를 활용한 시료 중의 주성분 정량 분석 58
5. Neochlorogenic acid, chlorogenic acid, 4-O-caffeoylquinic acid의 항산화 활성 61
(1) Neochlorogenic acid, chlorogenic acid, 4-O-caffeoylquinic acid의 DPPH radical 소거능 61
(2) Neochlorogenic acid, chlorogenic acid, 4-O-caffeoylquinic acid의 nitric oxide 생성억제 활성 64
6. Neochlorogenic acid, chlorogenic acid, 4-O-caffeoylquinic acid의 탈과립억제 효과 68
(1) RBL-2H3 세포의 세포생존율 68
(2) β-Hexosaminidase 방출억제 효과 70
참고문헌 72
Abstract 82
Table 1. LC condition for analysis of Eriobotrya japonica leaf extract 30
Table 2. Mass spectroscopy condition for analysis of major peaks separated... 31
Table 3. Yields of extract from Eriobotrya japonica leaf 35
Table 4. DPPH radical scavenging ability of Eriobotrya japonica leaf extract 40
Table 5. Multiple reaction monitoring parameters of LC-MS/MS analysis 59
Table 6. Contents of compounds isolated from Eriobotrya japonica leaf... 60
Table 7. DPPH radical scavenging ability of neochlorogenic acid, chlorogenic... 63
Figure 1. Flow chart of subculture for Raw 264.7 cell. 23
Figure 2. Flow chart of subculture for RBL-2H3 cell. 26
Figure 3. Flow chart of β -hexosaminidase release rate experiment in RBL-2H3... 28
Figure 4. Retention time confirmation and calibration curves of standard... 32
Figure 5. DPPH radical scavenging ability of water extract of Eriobotry... 38
Figure 6. DPPH radical scavenging ability of ethanol extract of Eriobotrya... 39
Figure 7. Cell viability of Eriobotrya japonica leaf water extract in LPS... 42
Figure 8. Cell viability of Eriobotrya japonica leaf ethanol extract in LPS... 43
Figure 9. Nitric oxide synthetic inhibit effect of Eriobotrya japonica leaf... 45
Figure 10. Nitric oxide synthetic inhibit effect of Eriobotrya japonica leaf... 46
Figure 11. Effects of Eriobotrya japonica leaf water extract on the... 48
Figure 12. Effects of Eriobotrya japonica leaf ethanol extract on the... 49
Figure 13. Effects of Eriobotrya japonica leaf water extract on the release rate... 52
Figure 14. Effects of Eriobotrya japonica leaf ethanol extract on the release... 53
Figure 15. Korean Eriobotrya japonica leaf water extract LC profile... 55
Figure 16. Negative mode LC-MS spectrum of major peaks of Korean... 56
Figure 17. Negative mode LC-MS spectrum of standard compounds. 57
Figure 18. DPPH radical scavenging ability of neochlorogenic acid,... 62
Figure 19. Cell viability of neochlorogenic acid, chlorogenic acid and... 65
Figure 20. Nitric oxide synthesis inhibit effect of neochlorogenic acid,... 67
Figure 21. Effects of neochlorogenic acid, chlorogenic acid and 4-O-caffeoyl-... 69
Figure 22. Effects of neochlorogenic acid, chlorogenic acid, 4-O-caffeoylquinic... 71