표제지
목차
요약 7
I. 서론 9
II. 재료 및 방법 11
1. 재료 11
1) 세포 11
2) 약재 11
3) 동물 및 사료 11
4) 시약 12
5) 기기 13
2. 방법 13
1) 시료 추출 13
2) RAW 264.7 cell의 배양 13
3) 세포 독성 14
4) 항산화 효농 14
5) 항염증 효능 14
6) 경골 골절 유발 16
7) 그룹 배정 및 시료 처리 16
8) 혈청 분리 17
9) 골절유합 관련 인자 측정 17
10) 방사선 검사 18
3. 통계처리 18
III. 결과 20
1. 안전성 20
1) RAW 264.7 cell에 대한 세포 독성 20
2. 항산화 효능 22
1) DPPH radical 소거능 22
3. 항염증 효능 24
1) NO 생성량 24
2) PGE₂ 생성량 26
3) Cytokine 생성량 28
4. 골절유합 관련 인자 측정 34
1) Osteocalcin 생성량 34
2) Calcitonin 생성량 36
3) CTXII 생성량 38
4) TGF-β 생성량 40
5) BMP-2 생성량 42
5. 방사선 소견 44
IV. 고찰 47
V. 결론 55
참고문헌 57
Abstract 65
Table I. The Compositions of Gyejibokryunghwan Extracts 11
Fig. 1. Cell viability of GBH extract in RAW 264.7 cells. 21
Fig. 2. DPPH free radical scavenging activity of GBH extract at... 23
Fig. 3. Effect of GBH extract on LPS-induced NO production in RAW... 25
Fig. 4. Effect of GBH extract on LPS-induced PGE₂ production in RAW... 27
Fig. 5. Effect of GBH extract on LPS-induced IL-lβ production in... 29
Fig. 6. Effect of GBH extract on LPS-induced IL-6 production in RAW... 31
Fig. 7. Effect of GBH extract on LPS-induced TNF-α production in... 33
Fig. 8. Effect of GBH on the level of osteocalcin in the serum of tibia-... 35
Fig. 9. Effect of GBH on the level of calcitonin in the serum of tibia-... 37
Fig. 10. Effect of GBH on the level of CTXII in the serum of tibia-... 39
Fig. 11. Effect of GBH on the level of TGF-β in the serum of tibia-... 41
Fig. 12. Effect of GBH on the level of BMP-2 in the serum of tibia-... 43
Fig. 13. Image of bone regeneration using X-ray in control group. 45
Fig. 14. Image of bone regeneration using X-ray in GBH group. 46