표제지
요약
목차
I. 서론 8
II. 재료와 방법 10
1. 플라스미노겐 정제 (Plasminogen purification) 10
2. 재조합 단백질의 정제 (Purification of recombinant proteins) 10
3. αX I-domain 돌연변이 단백질 정제 (Purification of αX I-domain mutant proteins) 10
4. Peptides 11
5. 고체상 결합 분석 (Solid phase binding assay) 11
6. 표면 플라스온 공명 (Surface plasmon resonance) 분석 12
III. 결과 13
IV. 논의 20
V. 참고문헌 24
Abstract 28
Table 1. Kinetics of αX I-domain binding to immobilized plasminogen. 19
Fig.1. SPR sensorgrams of GST-αX I-domain binding to plasminogen. 15
Fig.2. Binding analyses of loop-specific peptides to plasminogen. Each peptide (1.0 mM)... 16
Fig.3. Binding of mutant proteins dervied from the βD-α5 loop of the αX I-domain to... 17
Fig.4. Inhibition of GST-αX I-domain binding to plasminogen by an excess of lysine... 18
Fig.5. Crystal structure of the αX I-domain with critical amino acid residuses for... 23