Title Page
Contents
Abstract 8
Introduction 10
Materials and Methods 14
2.1. Bacterial Strain and Culture 14
2.2. Sample collection, Phage Isolation and Purification 15
2.3. Electron Microscopy of Phage 21
2.4. Host Range Determination 21
2.5. Stability at different pH and Temperature 23
2.6. One-Step Growth Curve o 23
2.7. Phage DNA Isolation 24
2.8. Whole genome sequence analysis 25
2.9. Challenge test and estimation of lethal dose 25
2.10. Phage treatment of infected fish 26
Results 27
3.1. Isolation of Bacteriophage 27
3.2. Host Range 29
3.3. Temperature and pH stability 32
3.4. One step growth curve 35
3.5. Genome analysis 36
3.6. Protective effect of AKh-2 in infected loach 36
Discussion 39
Conclusion 44
References 45
Table 1. Description of different strains of A. hydrophila used in the study 14
Table 2. Bacterial species used for determination of host range 22
Table 3. Host Range of bacteria 30
Fig. 1. Location of Geoje Island from where samples were collected 16
Fig. 2. Description of sampling site 17
Fig. 3. Plaque for isolation and titer determination of phage 20
Fig. 4. Electron micrograph of Akh-2 28
Fig. 5. Temperature stability of Akh-2 33
Fig. 6. pH stability of Akh-2 34
Fig. 7. One Step Growth Curve 35
Fig. 8. Protective effects of Phage 38