Title Page
Contents
Abstract 8
List of Abbreviations 10
Introduction 11
Materials and Methods 17
2.1. Study Site: Geographical Location 17
2.1.1. Sample collection and Preservation 19
2.2. Genomic DNA extraction 19
2.2.1. PCR amplification and sequencing 21
2.2.2. Assembly of mitochondrial genome by the bioinformatics analysis 22
Results and Discussion 23
3.1. Morphological and molecular Identification of Cynoglossus senegalensis and Chrysichthys nigrodigitatus. 23
3.2. Complete mitochondrial genome of Cynoglossus senegalensis. 26
3.3. Organization of full length mitochondrial genome of Cynoglossus senegalensis 29
3.4. Structure of tRNA genes of Cynoglossus senegalensis 32
3.5. Phylogenetic tree of Cynoglosus senegalensis 34
3.6. The complete mitochondrial genome of Chrysichthys nigrodigitatus 37
3.7. Organization of full length mitochondrial genome of Chrysichthys nigrodigitatus. 39
3.8. Structure of tRNA genes of Chrysichthys nigrodigitatus. 41
3.9. Phylogenetic tree of Chrysichthys nigrodigitatus 43
Conclusion 46
References 48
Table 1. List of Species specific primers used during this study 20
Table 2. Organization of the full-length mitochondrial genome of Cynoglossus senegalensis 31
Table 3. Organization of the mitochondrial genome of Chrysichthys nigrodigitatus 40
Figure 1. Map of Cameroon Coastline 18
Figure 2. Cynoglossus senegalensis (A) and Chrysichthys nigrodigtatus (B) collected from... 25
Figure 3. Mitochondrial genomic organization of Cynoglossus senegalensis 28
Figure 4. Structure of 22 tRNA genes in the mitochondrial genome of Cynoglossus senegalensis 33
Figure 5. Phylogenetic tree of Cynoglossus senegalensis 36
Figure 6. Mitochondrial genomic organization of Chrysichthys nigrodigitatus 38
Figure 7. Structure of 22 tRNA genes in Mitochondrial genome of Chrysichthys... 42
Figure 8. Phylogenetic tree of Chrysichthys nigrodigitatus within Order Siluriformes 45