Title Page
Contents
Abstract 8
1.0. Introduction 10
2.0. Materials and Methods 16
2.1. Microorganisms 16
2.2. Preparation of Nile perch fish wastewater 16
2.3. Adaptation of the microbial consortium 18
2.4. Biodegradation of Nile perch wastewater 20
2.5. Analysis 22
2.6. Bioactivities of the biodegraded Nile perch wastewater 22
2.6.1. 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity 22
2.6.2. 2, 2- Azino- bis3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay 23
2.6.3. Compounds contributing to Antioxidant Activity 25
2.7. Antimicrobial activity of the biodegraded NPWW supernatant 26
2.8. Statistical analysis 27
3.0. Results and Discussion 28
3.1. Biodegradation of Nile perch wastewater 28
3.2. Antioxidant activity of the biodegraded NPWW 32
3.3. Antimicrobial activity of NPWW supernatant 40
3.4. Compounds contributing bioactivity 43
4.0. Conclusion 47
5.0. Recommendation 48
References 51
Table 1. The amount of amino acids present in the soluble skin collagen and bone gelatin... 17
Table 2. List of high antioxidant activities of fishery hydrolysates and wastewater 39
Table 3. The MICs of NPWW supernatants and their antimicrobial susceptibilities... 42
Figure 1. Adaptation of the inoculum into sterile Nile perch wastewater prior to... 19
Figure 2. Sketch of an overall ecofriendly treatment process of the Nile perch wastewater 21
Figure 3. Trends of the parameters during the biodegradation of NPWW. The Data... 31
Figure 4. DPPH scavenging activity of the broth supernatants collected during... 35
Figure 5. ABTS cation decolorization assay of the culture supernatant collected during... 38
Figure 6. Amino acids profiles present in the biodegraded Nile perch supernatants... 46