Title Page
Contents
I. INTRODUCTION 12
1. Background information 12
2. Importance of research 15
3. Purpose of research 16
II. MATERIALS 17
1. Samples 17
2. Materials 17
III. METHODS 18
1. Physicochemical study 18
1.1. Proximate composition 18
1.2. Total phenol content and antioxidant capacity 22
1.3. Volatile compounds 23
2. Animal study 24
2.1. Animals and breeding condition 24
2.2. Chronic mild stress(CMS) 25
2.3. Inhalation condition 28
2.4. Behavior test 30
2.5. Blood pressure 32
2.6. Sacrifice 32
2.7. Serum and tissue analysis 32
3. Clinical study 36
3.1. Subjects and measurement condition 36
3.2. Electroencephalography(EEG) 37
3.3. Blood pressure and pulse 39
3.4. Cortisol 39
4. Statistical analysis 39
IV. RESULTS AND DISCUSSION 41
1. Physicochemical study 41
1.1. Proximate composition 41
1.2. Total phenol content and antioxidant capacity 44
1.3. Volatile compound 47
2. Animal study 58
2.1. Food intake and weight 58
2.2. Behavior test 62
2.3. Blood pressure 66
2.4. Tissue weight 70
2.5. Serum and tissue analysis 72
3. Clinical study 78
3.1. Electroencephalography(EEG) 78
3.2. Blood pressure and pulse 95
3.3. Cortisol 99
V. REFERENCES 101
ABSTRACT 111
국문초록 114
Table 1. Behavioral characteristics according to brain waves. 14
Table 2. Analysis condition of constituent amino acids and free amino acids. 21
Table 3. A weekly plan of animal experiments. 27
Table 4. Proximate compositions in Ocimum basilicum. 42
Table 5. Amino acids in Ocimum basilicum. 43
Table 6. Total phenol contents and antioxidant capacity in basil essential oil. 46
Table 7. Volatile compounds in Ocimum basilicum. 49
Table 8. Volatile compounds in basil essential oil. 53
Table 9. Measurement of volatile patterns in Ocimum basilicum and basil essential oil by... 57
Table 10. Changes of food intake amounts in animal groups. 59
Table 11. Changes of weight in animal groups. 60
Table 12. Dietary efficiency ratio in animal groups. 61
Table 13. Changes of sucrose intake amounts in animal groups. 63
Table 14. Changes of forced swimming test record in animal groups. 65
Table 15. Changes of systolic pressure in animal groups. 67
Table 16. Changes of diastolic pressure in animal groups. 68
Table 17. Changes of pulse in animal groups. 69
Table 18. Tissue weight in animal groups. 71
Table 19. Cholesterol and triglyceride amounts in serum in animal groups. 73
Table 20. Glutamic pyruvic transaminase and glutamic oxaloacetic transaminase amounts in... 75
Table 21. Cortisol and serotonin amounts in serum and brain tissue in animal groups. 77
Table 22. Changes of absolute theta wave by inhalation of basil essential oil. 79
Table 23. Changes of relative theta wave by inhalation of 1% basil essential oil. 80
Table 24. Changes of absolute alpha wave by inhalation of 1% basil essential oil. 83
Table 25. Changes of relative alpha wave by inhalation of 1% basil essential oil. 84
Table 26. Changes of A value by inhalation of 1% basil essential oil. 86
Table 27. Changes of absolute beta wave by inhalation of 1% basil essential oil. 88
Table 28. Changes of relative beta wave by inhalation of 1% basil essential oil. 89
Table 29. Changes of absolute gamma wave by inhalation of 1% basil essential oil. 92
Table 30. Changes of relative gamma wave by inhalation of 1% basil essential oil. 93
Table 31. Changes of systolic pressure by inhalation of 1% basil essential oil. 96
Table 32. Changes of diastolic pressure by inhalation of 1% basil essential oil. 97
Table 33. Changes of pulse by inhalation of 1% basil essential oil. 98
Table 34. Changes of cortisol by inhalation of 1% basil essential oil. 100
Figure 1. The diagram of chronic mild stress and behavior test. 26
Figure 2. Inhalation of the volatile compounds in 1% basil essential oil to rats. 29
Figure 3. Forced swimming test. 31
Figure 4. The international 10-20 system. 38
Figure 5. Total ion chromatogam of volatile compounds in Ocimum basilicum. 52
Figure 6. Total ion chromatogram of volatile compounds in basil essential oil. 56
Figure 7. Changes of food intake amounts in animal groups. 59
Figure 8. Changes of weight in animal groups. 60
Figure 9. Changes of 1% sucrose intake amounts in animal groups. 63
Figure 10. Changes of forced swimming test record in animal groups. 65
Figure 11. Changes of systolic pressure in animal groups. 67
Figure 12. Changes of diastolic pressure in animal groups. 68
Figure 13. Changes of pulse in animal groups. 69
Figure 14. Tissue weight in animal groups. 71
Figure 15. Cholesterol and triglyceride amounts in serum in animal groups. 73
Figure 16. Glutamic pyruvic transaminase and glutamic oxaloacetic transaminase amounts in... 75
Figure 17. Cortisol and serotonin amounts in serum and brain tissue in animal groups. 77
Figure 18. Brain mapping of theta wave by inhalation of 1% basil essential oil. 81
Figure 19. Brain mapping of alpha wave by inhalation of 1% basil essential oil. 85
Figure 20. Brain mapping of beta wave by inhalation of 1% basil essential oil. 90
Figure 21. Brain mapping of gamma wave by inhalation of 1% basil essential oil. 94