표제지
목차
I. 서론 10
II. 연구사 12
1. 닭의 생식기관의 조직학적 특성 12
2. 난각의 형성기전 13
3. 난각의 구성 물질 16
4. 연령에 따른 난각 형성 관련 체내 생리적 변화 및 난각의 변화 18
5. 연령 및 난각 관련 유전자 20
1) Telomere 20
2) 난각 단백질 관련 유전자 20
3) 난각 이온 관련 유전자 24
III. 재료 및 방법 29
1. 공시동물 및 분석 시료 29
2. qRT-PCR을 이용한 telomere 및 난각 형성관련 유전자 분석 30
1) RNA 추출, cDNA 합성 및 primer 제작 30
2) Quantitative Real-time PCR 수행 30
3. 혈중 이온분석 32
4. 조직표본 제작 및 염색 32
1) 조직표본을 위한 Paraffin Block 제작 32
2) 염색 33
3) 분석 33
5. 난각 분석 35
1) FE-SEM을 이용한 난각 분석 35
2) ICP를 이용한 난각 구성성분 분석 35
6. 통계분석 36
IV. 결과 및 고찰 37
1. 연령에 따른 혈중 이온 분석 37
2. 연령에 따른 자궁조직의 변화 40
3. 연령에 따른 난각 미세구조의 변화 47
4. 연령에 따른 난각 구성이온의 변화 56
5. 연령에 따른 난각 관련 유전자들의 발현 양상 59
V. 결론 65
VI. 참고문헌 67
VII. 국문초록 83
ABSTRACT 85
Table 1. Formula of the basal diets fed to laying hen 29
Table 2. The primers of telomere and eggshell related genes for qRT-PCR 31
Table 3. The concentration of ion component in the blood of laying hens at different... 39
Table 4. Comparison of cilia length of uterine epithelial cell according to hen age 46
Table 5. Comparison of eggshell ultrastructure parameters according to hen age 55
Table 6. Comparison of the contents of eggshell ion components according to hen age 58
Table 7. The correlation coefficients among eggshell protein genes 63
Table 8. The correlation coefficients among eggshell ion related genes 64
Table 9. The correlation coefficients between eggshell protein genes and ion related... 64
Figure 1. Composition of eggshell calcified layers and distribution of eggshell matrix... 23
Figure 2. The pathway of uterine ion transporters and ion related genes expression in... 28
Figure 3. Preparation of Paraffin blocks and Hematoxylin-Eosin stain. 34
Figure 4. Uterine cilia epithelial cells and uterus endometrial tissues in laying hens at... 42
Figure 5. Microscopical morphology of uterine cilia epithelial cells and uterus... 43
Figure 6. Microscopical morphology of uterine cilia epithelial cells and uterus... 44
Figure 7. Microscopical morphology of uterine cilia epithelial cells and uterus... 45
Figure 8. The microscopical morphology of uterine cilia epithelial cells in laying hens... 46
Figure 9. Cross sectional length of palisade and mammillary layer in eggshells... 49
Figure 10. The patterns of mammillary knobs density and mammillary bodies in the... 50
Figure 11. The patterns of mammillary knobs density and mammillary bodies in the... 51
Figure 12. The patterns of mammillary knobs density and mammillary bodies in the... 52
Figure 13. Comparison of mammillary knobs density and mammillary bodies in the egg-shells according to hen age. 53
Figure 14. Ultrastructure of abnormal mammillary bodies(type B) and sponge structure... 54
Figure 15. The cuticle surface of egg-shell according to hen age. 54
Figure 16. The amount of telomeric DNA in chicken uterus cells according to hen... 62
Figure 17. The expression values of eggshell protein genes in chicken uterus cells... 62
Figure 18. The expression values of eggshell related ion genes in chicken uterus cells... 63
Figure 19. Effect of hen aging on the mechanism of eggshell formation. 66