Title Page
Contents
Abbreviations 7
I. Introduction 8
II. Materials and Methods 11
Animals and phenotypes 11
Tissue expression of the BRD2 gene 12
BRD2 SNP detection and genotyping 13
Statistical analysis 16
III. Results and Discussion 17
Tissue expression profile of BRD2 mRNA 17
Association between an nsSNP in BRD2 and meat quality 19
IV. Conclusions 29
V. References 30
초록 35
Abstract 38
Table 1. Oligonucleotides used for genotyping 15
Table 2. Genotypes and allele frequencies of the BRD2 gene c.1709G>C nsSNP in the... 21
Table 3. Association between BRD2 nsSNP (c.1709G>C) and meat quality traits 23
Table 4. Association between BRD2 nsSNP (c.1709G>C) and meat quality traits in barrow 24
Table 5. Association between BRD2 nsSNP (c.1709G>C) and meat quality traits in gilt 25
Figure 1. Structure of the porcine BRD2 gene and the protein encoded by the gene 10
Figure 2. The porcine BRD2 mRNA expression was determined in various tissues by RT-... 18
Figure 3. The location of the porcine BRD2 gene c.1709G>C nsSNP and functional amino... 20
Figure 4. Hypothesis of action for nsSNP in porcine BRD2 gene 28