Title Page
Contents
Abbreviations 6
I. Introduction 7
II. Materials and Methods 10
Animals 10
Analysis of meat quality traits 10
RNA-Sequencing 11
Genotype analysis 12
Cell culture and pre-adipocyte differentiation 15
Oil Red-O staining 15
Real-time quantitative polymerase chain reaction (qPCR) analysis 16
Reverse transcription polymerase chain reaction (RT-PCR) analysis 19
Statistical analysis 19
III. Result and Discussion 20
Analysis of the association between meat quality traits and genotype 20
CIDE-B mRNA expression during adipogenesis 30
Relative CIDE-B expression levels in various tissues of the Berkshire pig 33
IV. Conclusion 36
V. References 38
초록 43
Abstract 46
Table 1. Summary for analysis of genotypes. 13
Table 2. Primers for real-time qPCR and RT-PCR analyses. 18
Table 3. Summary of the CIDE-B SNP c.414G>A analysis. 21
Table 4. Analysis of the association between general characteristics of meat-quality and CIDE-B synonymous SNP c.414G>A. 22
Table 5. Analysis of the association between meat moisture characteristics and CIDE-B synonymous SNP c.414G>A. 24
Table 6. Analysis of the association between meat chemical composition and CIDE-B synonymous SNP c.414G>A. 25
Table 7. Analysis of the association between post-mortem meat pH and temperature and CIDE-B synonymous SNP c.414G>A. 26
Table 8. Association between pork quality traits in gilts and CIDE-B synonymous SNP c.414G>A. 28
Table 9. Association between pork quality traits in barrows and CIDE-B synonymous SNP c.414G>A. 29
Figure 1. Position of oligonucleotides on genomic DNA analysis. 14
Figure 2. CIDE-B mRNA expression during adipogenesis. 31
Figure 3. mRNA expression of Adipogenic marker gene genes during adipogensis 32
Figure 4. qPCR analysis of CIDE-B in various tissues of the Berkshire pig. 35
Figure 5. Hypothesis of action for the CIDE-B gene SNP. 37