표제지
목차
I. 서론 10
II. 연구사 12
1. 닭의 성감별 12
2. 조우성과 만우성의 특징 14
3. 닭의 깃털형태와 발생 17
III. 재료 및 방법 20
1. 공시동물 20
2. 깃털 발생 및 형태 분석 20
3. 조우성과 만우성 생산능력 분석 20
4. 조우성과 만우성 개체 식별을 위한 분자생물학적 검정 21
1) 시료 채취 21
2) Genomic DNA 추출 21
3) 만우성 특이 유전자를 이용한 PCR 및 전기영동 21
5. 통계분석 23
IV. 결과 및 고찰 24
1. 조우성과 만우성 병아리의 깃털 발생 양상과 형태 분석 24
1) 발생 병아리의 날개깃의 형태 분석 24
2) 조우성과 만우성 개체의 날개깃 발달 양상 비교 분석 28
3) 조우성과 만우성 개체의 꼬리깃 발달 양상 비교 분석 38
2. 만우성 특이 유전자를 이용한 깃털 조만성 개체 확인 43
3. 공시 집단 내 조우성과 만우성 개체의 분포 분석 45
4. 조우성과 만우성 개체의 생산능력 비교 47
V. 결론 49
VI. 참고문헌 51
국문초록 57
ABSTRACT 59
Table 1. The PCR primers for K-specific gene 22
Table 2. The conformity of phenotypic classification and PCR classification for... 44
Table 3. The number of chicks and distribution frequency of early- and... 46
Table 4. The genotype frequency of feathering gene in Korean native chicken 46
Fig. 1. Chicken wing feather 25
Fig. 2. The patterns of wing feather of early-feathering and late-feathering at... 26
Fig. 3. The diagram of wing feathers types in early-feathering and late-feathering. 27
Fig. 4. The number of chicks and distribution according to the feathering types in... 27
Fig. 5. The developmental patterns of wing feathers in early-feathering chickens. 31
Fig. 6. The development of wing feathers length in early-feathering chicken from day-old to 55-day-old. 32
Fig. 7. The developmental patterns of wing feathers in LF-Less, LF-Scant, LF- Equal and LF-Reverse types. 33
Fig. 8. The development of wing feather length in LF-Less, LF-Scant, LF-Equal and LF-Reverse types from day-old to... 34
Fig. 9. The development of pimaries length in early-feathering chicken and... 35
Fig. 10. The comparison of primaries length from day-old to 55-day-old with... 35
Fig. 11. The development of coverts length from day-old to 55-day-old in... 36
Fig. 12. The comparison of coverts length from day-old to 55-day-old with... 36
Fig. 13. The change of wing feather length from 1-day-old to 55-day-old in... 37
Fig. 14. The patterns of tail feather from day-old to 15-day-old in early-feathering chickens. 39
Fig. 15. The patterns of tail feather from day-old to 17-day-old in late-feathering chickens. 40
Fig. 16. The patterns of tail feathers in early-feathering chicken(EF) and late-feathering(LF) chicken. 41
Fig. 17. The comparison of developmental patterns of tail feather between early-feathering(EF) and late-feathering(LF)... 42
Fig. 18. The results from polymerase chain reaction using the specific primer of... 44
Fig. 19. Body weights of early-feathering(EF) and late-feathering(LF) chicken... 48
Fig. 20. Viability of early-feathering(EF) and late-feathering(LF) chicken from... 48