Title Page
Contents
Abbreviations 6
I. Introduction 7
II. Materials and Methods 10
Animals 10
Analysis of AGPAT5 expression by RT-PCR 10
AGPAT5 SNP detection and genotyping 11
Measurements of meat quality traits 13
Statistical analysis 14
III. Results 15
AGPAT5 expression in various tissues of the Berkshire pig 15
Identification of an nsSNP in AGPAT5 and genotyping 17
Association of the AGPAT5 nsSNP with meat quality traits 19
IV. Discussion 23
V. Conclusion 24
VI. References 26
초록 30
Abstract 32
Table 1. Oligonucleotides used for SNP detection and RT-PCR. 12
Table 2. Genotype and allele frequencies of non-synonymous SNP in AGPAT5 gene. 18
Table 3. Association between AGPAT5 nsSNP, c.677A>G, and meat quality traits. 20
Figure 1. Biosynthetic pathway of AGPAT5 gene. 9
Figure 2. The AGPAT5 mRNA expression was determined in various tissues by RT-PCR. 16
Figure 3. Hypothesis of action for AGPAT5 gene nsSNP. 25