Title Page
Contents
Abstract 10
1. INTRODUCTION 12
1.1. Background study 12
2. MATERIALS AND METHOD 15
2.1. Source of experimental larvae and rearing conditions 15
2.2. Experimental design 16
2.3. Experimental biofloc water preparation 17
2.4. Biofloc management 17
2.5. Feeding of rock worm larvae 18
2.6. Physiochemical biofloc water quality analysis 18
2.7. Data collection 23
2.8. Statistical analysis 23
3. RESULTS 24
3.1. Survival rate of M. sanguinea larvae 24
3.2. Growth performance of M. Sanguinea larvae 27
3.3. Biofloc water physiochemical quality parameters 31
4. Discussion and Conclusion 44
References 49
Table 1. Survival rate and the final weight of M. sanguinea indifferent densities by... 32
Table 2. Results of one-way random effects ANOVA test for final number of worms on... 33
Table 3. Concentration of biofloc water quality parameters, by biofloc technology used... 34
Fig. 1. Morphology of Marphysa sanguinea larvae (trochophore) used indetermining the... 15
Fig. 2. Schematic diagram of the biofloc technology system experiment set-up. 19
Fig. 3. Experimental set-up of biofloc technology system, showing arrangement of... 20
Fig. 4. Structure of sediments used in the rearing bottle for Marphysa sanguinea larvae in... 21
Fig. 5. Biofloc 'floc' particles from experimental biofloc water used for rearing Marphysa... 21
Fig. 6. Apparatus used for checking biofloc water quality parameters. 22
Fig. 7. Comparison on survival rate of M. Sanguinea larvae at different density groups in... 25
Fig. 8. Comparison on final number worms of M. sanguinea larvae at different density... 26
Fig. 9. Comparison of weights in different density groups by biofloc technology for M.... 28
Fig. 10. Comparison between survival rate and final means weight on different densities... 29
Fig. 11. Comparison between number segments of M. sanguinea larvae in biofloc... 30
Fig. 12. Trend of ammonia NH₃ (㎎/L) in biofloc water used for rearing Marphysa... 35
Fig. 13. Trend of nitrite NO₂ (㎎/L) concentration in biofloc water used for rearing... 36
Fig. 14. Trend of nitrate NO₃ (㎎/L) concentration in biofloc water for rearing Marphysa... 37
Fig. 15. Trend of alkalinity (CaCO₃) in biofloc water for rearing Marphysa sanguinea... 38
Fig. 16. Trend of pH (㎎/L) in biofloc water for rearing Marphysa sanguinea larvae. 39
Fig. 17. Trend of temperature (℃) in biofloc water for rearing Marphysa sanguinea... 40
Fig. 18. Trend of dissolved oxygen concentration (㎎/L) in biofloc water for rearing... 41
Fig. 19. Trend of salinity (psu) in biofloc water for rearing Marphysa sanguinea larvae. 42
Fig. 20. Trend of total suspension solids (㎎/L) in biofloc water for rearing Marphysa... 43