Title Page
Contents
Abstract 8
Chapter 1. Introduction 10
Chapter 2. Materials and Methods 14
2.1. Experimental Design and Diets 14
2.2. Fish and Feeding Protocol 16
2.3. Sample Collection and Analysis 17
2.4. Statistical Analysis 19
Chapter 3. Results 20
3.1. Growth Performances 20
3.2. Non-specific Immune Responses 21
3.3. Whole Body Proximate Analysis 21
3.4. Haematological Parameters 22
Chapter 4. Discussion and Conclusions 38
References 46
Appendices 7
Appendix 1. Initial weight of experimental juvenile rainbow trout for the 8 different experimental diet groups. 57
Appendix 2. Weight gain data of juvenile rainbow trout fed with 8 different experimental diets by the end of 4 weeks. 58
Appendix 3. Final weight gain data of juvenile rainbow trout fed with 8 different experimental diets for 7 weeks. 59
Appendix 4. Data on serum parameters of juvenile rainbow trout fed with 8 different experimental diets for 7 weeks. 60
Appendix 5. CF, VSI and HSI data of juvenile rainbow trout fed with 8 different experimental diets for 7 weeks. 61
Appendix 6. Proximate composition data of juvenile rainbow trout fed with 8 different experimental diets for 7 weeks. 62
Table 1. Formulation and proximate composition of 8 different experimental diets 15
Table 2. Growth performances of juvenile rainbow trout fed with 8 different experimental... 23
Table 3. Non-specific immune responses of juvenile rainbow trout fed with 8 different... 29
Table 4. Whole body proximate composition of juvenile rainbow trout fed with 8... 32
Table 5. Haematological parameters of juvenile rainbow trout fed with 8 different... 33
Fig. 1. Weight gain of juvenile rainbow trout fed with 8 different experimental diets for 7... 24
Fig. 2. Specific growth rate of juvenile rainbow trout fed with 8 different experimental... 25
Fig. 3. Feed efficiency of juvenile rainbow trout fed with 8 different experimental diets... 26
Fig. 4. Protein efficiency ratio of juvenile rainbow trout fed with 8 different experimental... 27
Fig. 5. Survival rate of juvenile rainbow trout of 8 different experimental diets... 28
Fig. 6. Serum lysozyme activity of juvenile rainbow trout fed with 8 different... 30
Fig. 7. Superoxide dismutase activity of juvenile rainbow trout fed with 8 different... 31
Fig. 8. Glucose activity in blood serum of juvenile rainbow trout fed with 8 different... 34
Fig. 9. Serum total protein contents of juvenile rainbow trout fed with 8 different... 35
Fig. 10. Aspartate aminotransferase (AST) activity of juvenile rainbow trout fed with... 36
Fig. 11. Alanine aminotransferase (ALT) activity of juvenile rainbow trout fed with 8... 37