Title Page
Contents
Abstract 8
Introduction 10
Materials and Methods 12
1. Quantification of indicator substance nicotinamide 12
2. Forelimb grip strength 13
3. Swimming endurance test 14
4. Weight Change 16
5. Biochemical assay 16
6. Statistical analysis 17
Results 18
1. Quantification of indicator substance nicotinamide 18
2. RP-HPLC quantification method of indicator substance nicotinamide validation 20
2.1. Linearity 20
2.2. Precision 22
2.3. Accuracy 22
3. Effect of nicotinamide and guanosine addition to protein in exercise performance. 23
Discussion 33
Conclusion 41
References 42
Table 1. Linearity, precision, and accuracy of RP-HPLC quantitation method for... 23
Table 2. Effect of best combination PRO+NAM+GUA on serum glucose, lactate, urea,... 32
Fig. 1. Detection and quantification of nicotinamide in RP-HPLC 13
Fig. 2. Mixture of hot water extract and reagent nicotinamide in RP-HPLC profile. 18
Fig. 3. Standard curve on RP-HPLC using semi-prep C18-column of the standard substance... 20
Fig. 4. Comparison of nicotinamide quantitation by dry weight of hot water extract with... 21
Fig. 5a. Relative increase day 0-7 on mice's body weight, forelimb test and swimming... 24
Fig. 5b. Relative increase day 0-7 on mice's body weight, forelimb test and swimming... 26
Fig. 6a. Relative increase day 0-7 on mice's body weight, forelimb test and swimming... 27
Fig. 6b. Relative increase day 0-7 on mice's body weight, forelimb test and swimming... 29
Fig. 7a. Relative increase day 0-7 on mice's body weight, forelimb test and swimming... 31
Fig. 7b. Relative increase day 0-7 on mice's body weight, forelimb test and swimming... 31