표제지
목차
I. 서론 10
II. 재료 및 방법 13
1. 산림토양으로부터 당화 박테리아 분리 13
2. 순수 분리된 당화 미생물의 동정 14
3. 분리된 당화 미생물의 효소활성 측정 16
III. 결과 및 고찰 17
1. 산림토양으로부터 배양 가능한 박테리아 및 목질바이오매스 분해 미생물의 비율 17
2. 목질바이오매스 분해 미생물의 분리 및 Vitek을 이용한 균 동정 21
3. 16S rRNA를 이용한 균의 동정 26
4. 박테리아 게놈의 목질바이오매스 분해 유전자 분석 32
5. 목질바이오매스 분해 미생물의 생장특성 46
6. CMCase와 Xylanase의 최적 온도 및 pH 51
7. CMCase와 Xylanase의 온도안정성 57
IV. 결론 62
참고문헌 64
국문초록 70
Abstract 72
Table 1. Physiological characteristics of Bacillus sp. EFL1-1 23
Table 2. Physiological characteristics of Bacillus sp. EFL2-1 24
Table 3. Physiological characteristics of Bacillus sp. EFP3-1 25
Figure 1. Representative plate photo for screening of cellulolytic bacteria from the soil... 20
Figure 2. Nucleotide sequence of 16S rRNA gene from Bacillus sp. EFL1 isolated... 28
Figure 3. Nucleotide sequence of 16S rRNA gene from Bacillus sp. EFL2 isolated... 29
Figure 4. Nucleotide sequence of 16S rRNA gene from Bacillus sp. EFP3 isolated... 30
Figure 5. A neighbor-joining phylogenetic tree based on the 16S rRNA gene of... 31
Figure 6. Nucleotide sequence and deduced amino acid sequence of BBt-glucanase-1... 34
Figure 7. Nucleotide sequence and deduced amino acid sequence of BBt-glucanase-2... 35
Figure 8. Nucleotide sequence and deduced amino acid sequence of BBt-cellulase-1 from... 36
Figure 9. Nucleotide sequence and deduced amino acid sequence of BBt-xylanase-1... 37
Figure 10. Nucleotide sequence and deduced amino acid sequence of BSB-glucanase-1... 38
Figure 11. Nucleotide sequence and deduced amino acid sequence of BSB-glucanase-2... 39
Figure 12. Nucleotide sequence and deduced amino acid sequence of BSB-xylanase-1... 40
Figure 13. Nucleotide sequence and deduced amino acid sequence of BSB-xylanase-2... 41
Figure 14. Nucleotide sequence and deduced amino acid sequence of BGQK-glucanase-1... 42
Figure 15. Nucleotide sequence and deduced amino acid sequence of BGQK-glucanase-2... 43
Figure 16. Nucleotide sequence and deduced amino acid sequence of BGQK-xylanase-1... 44
Figure 17. Nucleotide sequence and deduced amino acid sequence of BGQK-xylanase-2... 45
Figure 18. Cell growth and enzymatic activities of CMCase and xylanase produced by... 48
Figure 19. Cell growth and enzymatic activities of CMCase and xylanase produced by... 49
Figure 20. Cell growth and enzymatic activities of CMCase and xylanase produced by... 50
Figure 21. Effect of pH and temperature on the relative activity of CMCase (A) and... 54
Figure 22. Effect of pH and temperature on the relative activity of CMCase (A) and... 55
Figure 23. Effect of pH and temperature on the relative activity of CMCase (A) and... 56
Figure 24. Thermal stability of CMCase (A) and xylanase (B) from bacillus sp. EFL1. 59
Figure 25. Thermal stability of CMCase (A) and xylanase (B) from bacillus sp. EFL2. 60
Figure 26. Thermal stability of CMCase (A) and xylanase (B) from bacillus sp. EFP3. 61